福建省9个猪场大肠杆菌病原菌的分离鉴定

Isolation and identification of E. coli pathogens from 9 swine farms in Fujian Province

从福建省的福州、福清、漳州、莆田、邵武、厦门、泉州等9个发病猪场采集病料,共分离出14株病原菌,经福建省防疫站菌种鉴定和用大肠杆菌K88、K99的特异引物进行PCR扩增,结果表明所分离的大肠杆菌中 10株为普通大肠埃希氏菌,2株为β溶血性菌株。2株为大肠杆菌K88。纯化菌株的药敏试验结果表明,和大肠杆菌标准株K88、K99相比,不同猪场分离的大肠杆菌对抗菌素的耐药性都有较大增强。

Forteen strains of E. coli pathogens were isolated from 9 swine farms in Fuzhou, Fuqlng, Zhangzhou, Putian, Shaowu, Xiamen, Quanzhou of Fujian, and were identified by Fujian Epidemic Prevention Station and analyzed by PCR with E. coli K88 and K99 specific PCR primers. The result showed that 10 strains belonged to normal E. coli, 2 strains belonged to β- hemolytic E. coli and 2 strains belonged to E. coli K88. Insecticide tolerance of the isolated strains were higher than that of standard strains E. coli K88, K99 by drugsensitive test.