樱桃番茄种质资源遗传多样性的RAPD分析

Genetic diversity of Lycopersicon esculentum var. cerasiforme germplasms by RAPD analysis

从200个随机引物中筛选出27个引物,对32个樱桃番茄品种进行RAPD分析,并对樱桃番茄遗传多样性和分类进行探讨和研究,结果显示,27个引物共扩增出207条DNA谱带,其中多态条带139条,多态性程度为 67．15％;平均每个引物产生7．67条,每个引物可扩增出2-12条多态性谱带,谱带大小为270-2 000 bp;遗传距离D值在0．33水平上,能将供试材料聚为3类:野生种聚成一类,果皮为黄色和少数红色品种聚成一类,全部是红色聚成一类;此外,还发现从重要特征观察,樱桃番茄品种表现出一定的聚集趋势。

The genetic polymorphisms in Lycopersicon esculenturn var. cerasiforrae of thirty-two cultivars were investigated by using random amplified polymorphic DNA markers （RAPD）. Twenty-seven primers selected from two handred primers amplified 207 DNA fragments and 139 bands showed polymorphisms that occupied 67.15% of the whole amplified bands. Each primer could amplify 2 to 12 polymorphic bands, with an average of 7.67 bands. The length of most amplified fragments ranged from 270 to 2000 bp. Cluster analysis showed that the 32 cultivars could be classified into 3 groups at the level of D 0. 33, which evidently showed the variations in some important traits of 1.ycopersicon esculenturn var. cerasi forrne.