子宫内膜异位症大鼠血浆神经内啡肽特点及其对妊娠功能的影响

Changes of Plasma Endogenous Peptides and Its Effect on Gestational Function in Endometriosis Rats

【目的】探讨血浆β-内啡肽(-βEP)水平对子宫内膜异位症(EMT)大鼠妊娠功能的影响。【方法】将雌性SD大鼠于第3个动情周期的动情期复制EMT模型,取造模成功大鼠20只,随机分为模型组和那曲酮(为阿片受体拮抗剂)组各10只,另设假手术组大鼠10只;那曲酮组于动情前期灌服那曲酮2 mg.kg-1.d-1,其他时间灌服等容积生理盐水,模型组和假手术组均灌服生理盐水,连续1个月;取血检测动情前期血清-βEP、促黄体生成素(LH)、催乳素(PRL)水平,在动情后期检测血清孕激素(P)水平以及妊娠功能指标。【结果】模型组大鼠动情前期血清-βEP、PRL水平升高,LH水平降低,动情后期血清P水平降低,与假手术组比较均具有显著性差异(P<0.05或P<0.01);那曲酮组大鼠动情前期血清-βEP、PRL水平降低,LH水平升高,动情后期血清P水平升高,与模型组比较均具有显著性差异(P<0.05或P<0.01);妊娠功能各指标中那曲酮组大鼠排卵数、孕育活胎数、受孕率、着床率均较模型组显著升高(P<0.05或P<0.01)。【结论】EMT模型大鼠血清-βEP水平升高是其妊娠功能障碍的原因之一,阿片受体拮抗剂可改善生殖内分泌状态而有效提高妊娠功能。

[ Objective] To observe the effect of plasma β-endogenous peptides （β-EP） on gestational function in endometriosis （EMT） rats. [Methods] Female SD rats at the third estrous cycle were used to establish EMT model. Then 20 EMT rats were equally randomized into the model group and naltrexone （an opioid receptor antagonist） group, and other 10 rats were in the pseudo-operation group. Naltrexone group was given naltrexone 2 mg·kg^- 1· d^- 1 by gastric gavage during proestrus and normal saline at other time, and the model group and pseudo-operation group were given normal saline for one month. Serum levels of β-EP, luteinizing hormone （LH） and prolactin （PRL） were examined during proestrus, and serum progesterone （P） level and gestational function were observed after estrus. [ Results ] Serum levels of β-EP and PRL increased and LH decreased during proestrus, and serum P level also decreased after estrus in the model group （P 〈 0.05 or P 〈 0.01 compared with the pseudo-operation group）. β-EP and PRL levels decreased and LH increased during proestrus, and serum P level increased after estrus in naltrexone group （ P 〈 0.05 or P〈 0.01 compared with the model group）. Gestational functions such as ovulation number, gestational living fetus number, gestational rate and implantation rate were improved in naltrexone group （ P 〈 0.05 or P 〈 0.01 compared with the model group）. [ Conclusion] The increase of serum β-EP level is one of the factors of gestational disorder, and the opioid receptor antagonist can improve the endocrine function of EMT rats and promote the gestational function.