化橘红原植物化州柚生长过程中黄酮类成分的变化规律研究

Observation on Dynamic Changes of Flavonoids Contents of Citrus grandis(L.)Osbeck var.tomentosa Hort.

【目的】观测化橘红来源植物化州柚在生长过程中黄酮类等成分含量的动态变化规律,为确立采收化橘红时机提供依据。【方法】分别采用分光光度法、高效液相色谱法测定化州柚果皮和叶中总黄酮及柚皮苷含量,并制备指纹图谱。【结果】随着果龄的增加,化州柚果皮和叶中柚皮苷及总黄酮含量均呈显著性下降趋势;但指纹图谱显示,成果初期果皮中野漆树苷含量随果龄的增加而显著升高,62 d后才显著下降,叶中野漆树苷含量则无显著性变化。【结论】从经济价值和有效成分含量两方面综合考虑,果龄34 d时采收化州柚幼果,果龄55 d时采收化州柚未成熟果较为合适。

[ Objective] To observe the dynamic changes of flavonoids contents of Citrus grand/s （L.） Osbeck var. tomentosa Hort.. [ Methods] The total flavonoid content was determined by spectral photometric analysis and naringin content by HPLC, and the fingerprints of Citrus grandis （L.） Osbeck var. tomentosa Hort. were studied by HPLC. [Results] With the increase of the fruit age, total flavonoid contents in the peel and the leaves of Citrus grandis （L.） Osbeek var. tomentosa Hort. decreased obviously. Fingerprints results showed that at the early fruit age the rhoifolin content in the peel increased with the fruit age and began to decrease 62 days later, while the rhoifolin content in the leaves showed no changes. [ Conclusion ] In terms of active components contents and economic value, the optimal collecting time for Citrus grand/s （L.） Osbeck var. tomentosa Hort. is： young fruit, 34 days, and immature fruit, 55 days.