结肠癌P53蛋白表达与临床病理的意义

P53 Protein Expression in Colorecatal Cancer and Its Relationship to Clinicopathological Course and Prognosis

为了研究P53基因突变与结肠癌发生发展及预后的关系,对102例结肠癌、24例癌旁组织、16例正常结肠组织石蜡标本进行P53单抗的免疫组化染色.结果表明:结肠癌P53蛋白阳性率达70.6%,癌旁组织为20.8%,正常结肠组织中均无蛋白表达;阳性率间有显著的相关性(P<0.01),P53蛋白在高、中、低分化癌间阳性率有显著差异(阳性率分别为54.5%、72.7%、88.0%);在癌浸润深度肌层、浆膜层、浆膜外有显著差异(阳性率分别为53.8%、70.9%、90.5%);有无淋巴结转移的阳性率间亦有显著差异(为83.%、61.7%).P53蛋白的表达与结肠癌发生发展有关,表达强度与癌的分化程度、浸润深度、转移及预后有关.P53基因可作为评估患者预后的指标.

In order to study the relationship of P53 gene mutation with the occurrence and prognosis of colorecatal cancer. Immunohistochemical method is used to examine paraffinembedd samples of 102 cancer, 24 nommaligant peri - tumoral tissue and 16 normal colorecatal tissues for overexpression of the P53 geate product. The P53 protein expression was high in 70.6% of colorecatal cancer,20.8% positive in tissues close to cancer. Normal tissue of colon showed negative expression of P53 protein. The positive rate of P53 was significantly higher in cancer than adjacent tissues or normal tissue（ P 〈0.01）. It was significantly difference in well, moderately and poorly differentiated adenocaeinomas（54.5%, 72.7% and 88.0%）. The P53 protein is involved in the carcinogenesis if colorecatal cancer. The degree of P53 protein expession was significantly correlated with the degree of tumor cell differention ,invasion,metastasis and prognosis.