中药狼疮方对狼疮样BXSB小鼠脾细胞CD_(134)/CD_(134)L表达的影响

Effect of Lupus Recipe on Gene Expression and Protein Synthesis of CD_(134) and CD_(134) L in Spleen Cells of Lupus-prone BXSB Mice

目的:探讨CD134/CD134L共刺激信号在SLE发病机制中的可能作用及狼疮方对其表达的影响。方法:采用狼疮样BXSB小鼠模型,随机分为3组:狼疮方治疗组、泼尼松治疗组、空白模型组,每组6只,疗程10周。另设与BXSB小鼠同基因的正常C57BL/6小鼠6只为正常对照组。分别采集上述各组小鼠外周血和脾组织进行检测。结果:(1)与正常对照组比较,模型组BXSB小鼠的血清IgG和抗ds-DNA抗体水平和脾组织CD4+CD134+、CD8+CD134+、CD19+CD134L+、CD23+CD134L+的百分比及CD134、CD134L的mRNA拷贝数都显著增高(P<0.05或P<0.01)。(2)狼疮方治疗组或泼尼松治疗组的血清IgG、抗ds-DNA抗体水平和脾组织CD4+CD134+、CD8+CD134+、CD19+CD134L+、CD23+CD134L+的百分比及CD134、CD134L的mRNA拷贝数都显著低于模型组(P<0.05或P<0.01),而与正常对照组比较无统计学差异(P均>0.05)。结论:狼疮样BXSB小鼠存在CD134/CD134L的异常表达。狼疮方可减轻狼疮样小鼠高丙种球蛋白血症,减少体内自身抗体产生,对CD134/CD134L存在泼尼松相当的下调作用,抑制T、B细胞的异常活化,有效地治疗SLE。

Objective：To investigate the effects of lupus recipe on gene expression and protein synthesis of CD134 and CI134 L in splenic cells of lupus- prone BXSB mice. Methods：Eighteen male lupus- prone BXSB mice model were used in the experiment. The model mice were divided into three groups： un- treated model group, lupus reeipe（LR） - treated group, and prednisone treated group. Model mice were killed in 10 weeks. The control groups consisted of 6 syngenetie normal C57BL/6 male mice. Peripheral blood and spleen tissues were collected for the study. Results： （ 1 ）the serum levels of total IgG and anti - dsDNA antibody in un - treated model group were higher than that in other groups. And there were no differences among LR- treated group, prednisone- treated group and control group. （2）The positive cell percentages of CD4^＋CD134^＋、CD8^＋CD134^＋、CD19^＋CD134L^＋、CD23^＋ CD134L^＋ and the CDI34, CD134 mRNA expressions from un - treated model group were significantly higher than those from control groups（P〈 0.05, P〈 0.01 ）. （3）The LR or prednisone significantly reduced the positive cell percentages of CD4^＋CD134^＋、CD8^＋CD134^＋、CD19^＋CD134L^＋、CD23^＋ CD134L^＋ and the CD134,CD134L mRNA expressions in LR - treated group or prednisone - treated group（ P 〈 0.05, P 〈 0.01 comparing with model group）, while the difference between the treated group and the normal group was not significant. Conclusion：BXSB mice has abnormal expression of CE134 and CD134L. Lupus recipe reduces the serum levels of IgG and auto - antibody production and can treat systemic lupus erythematosus effectively.