免疫即迹法与分离人皮肤IIF洁检测类天疱疮抗体的比较研究

Comparative Study of the NaCl-split Skin to Immunoblotting in Detecting Bullous Pemphigoid Antibodies.

采用１ｍｏｌ／ＬＮａＣｌ分离正常人皮肤为底物的ＩＩＦ法和免疫印迹技术检测１２２例类天疱疮患者血清中抗基底膜抗体，比较研究上述两种方法对检测类天疱疮抗体的灵敏度及价值。结果分离皮肤ＩＩＦ法阳性８９例（７２．９５％）。免疫印迹法检测，与２３０ＫＤ、１８０ＫＤ和１６０ＫＤ分子量蛋白抗原结合阳性９２例（７５．４１％）。两法合用总阳性率为８３．６１％。分离皮肤ＩＩＦ法适合于常规采用，而免疫印迹法是前者的进一步补充。

Using IIF on 1M NaCI-split normal human skinand immunoblotting, we have examined 122 BP seraand compared the sensitivity and the value of the twomethods for the detection of circulating autoantibodiesin BP. Eighty-nine out of the 122 BP sera (72. 95%)contained anti-BMZ antibodies demonstrable by IIF onNaCI-split skin. Ninety-two out of the 122 BP sera(75. 41%) combined with the 230KD MW protein,orthe 180KD MW and the 160KD MW proteins. The total percentage of positive reaction reached 83. 61%when two methods were used in parallel. IIF on NaClsplit is preferable for routine detection of BP antibodies; however,immunoblotting may be used as a complementary assay.