P53基因蛋白在皮肤鳞癌中的表达

The Expression of P53 Oncoprotein in Squamous Cells Carcinoma.

应用免疫组化ＡＢＣ法，用多克隆抗体ＣＭＩ和单克隆抗体ＰＡｂ１８０１检测５０例皮肤鳞癌及癌旁上皮中Ｐ５３基因蛋白的表达。结果显示在正常上皮与癌旁轻、中度不典型增生上皮中未见Ｐ５３基因蛋白的表达，在１２．５％（２／１６）癌旁重度不典型增生上皮中和５６％（２８／５０）鳞癌中有Ｐ５３基因蛋白的表达，在分化差的鳞癌Ⅲ～Ⅳ级中有５５．５６％（５／９）的病例Ｐ５３阳性表达细胞超过１０％，相反分化较好的鳞癌Ⅰ～Ⅱ级中Ｐ５３阳性表达细胞少于１０％，提示Ｐ５３基因蛋白的表达与鳞癌发生密切相关，它是肿瘤进展为恶性的标志，并且在鳞癌进展阶段呈稳定增加。

We have examined the overexpression of the P53oncoprotein immunohistochemically in 50 cases sequamous cells carcinoma (SCC) of skin. P53 oncoproteinwas identified in 56% of SCC and 12. 5% of severedysplastic paraepithelium of carcinoma. In 55. 56%positive cases of Ⅲ￣Ⅳ carcinoma, more than 10%tumour cells were immumoreactive for P53 oncoprotein. No staining was found in normal tissue, slightand moderate dysplastic paraepithelium of carcinomaand the stroma cells. Our results indicate that there isa close relationship between the expression of P53 oncoprotein and SCC. It may be involved in the malignant transformation process of skin squamous cellscarcinoma and present steady increased in the developing stage of SCC.