Design of Vibrio 16S rRNA Gene Specific Primers and Their Application in the Analysis of Seawater Vibrio Community

类 Vibrio 原因 vibriosis 的病原的种， maricultured 动物和海味消费者的最流行的疾病之一。在海味生产的链监视他们的动力学，处理并且消费为食物和海洋生物养殖安全是很重要的。为了充实代表 Vibrio 16S ribosomal RNA 基因(rDNA ) ，碎裂并且识别进一步真实及时的这些细菌并且同时地在在链的细菌的植物群之中，有选择地放大 Vibrio 16S rDNA 碎片的一双教材与他们在海水 Vibrio 的分析证明的特性和报道被设计社区。二份教材， VF169 和 VR744 的 Thespecificities 和范围，被使用强风节目在在 GenBank 可得到的细菌的 16S rDNAs 之中理论上决定，实际上，由放大 Vibrio16S， rDNA 从海水 DNA 碎裂。超过 88.3% 序列在 GenBank，与 VR744 显示出相同火柴，属于 Vibrio 类。33 克隆的一个总数随机被选择并且定序。所有序列显示出他们的最高的类似到并且多样的已知的 Vibrio 种类在那些附近聚类。设计的教材能够检索宽范围 ofVibrio 16S rDNA 在海水在细菌的植物群之中明确地碎裂，海味耕作的最重要的自然环境。

The pathogenic species of genus Vibrio cause vibriosis, one of the most prevalent diseases of maricultured animals and seafood consumers. Monitoring their kinetics in the chain of seafood production, processing and consumption is of great importance for food and mariculture safety. In order to enrich Vibrio-representing 16S ribosomal RNA gene （rDNA） fragments and identify these bacteria further real-timely and synchronously among bacterial flora in the chain, a pair of primers that selectively amplify Vibrio 16S rDNA fragments were designed with their specificities and coverage testified in the analysis of seawater Vibrio community. The specificities and coverage of two primers, VF169 and VR744, were determined theoretically among bacterial 16S rDNAs available in GenBank by using BLAST program and practically by amplifying Vibrio 16S rDNA fragments from seawater DNA. More than 88.3% of sequences in GenBank, which showed identical matches with VR744, belong to Vibrio genus. A total of 33 clones were randomly selected and sequenced. All of the sequences showed their highest similarities to and clustered around those of diverse known Vibrio species. The primers designed are capable of retrieving a wide range of Vibrio 16S rDNA fragments specifically among bacterial flora in seawater, the most important natural environment of seafood cultivation.