2例人类免疫缺陷病毒Ⅰ型母婴传播病例的毒株序列分析

Sequence analysis of HIV-1 strains from 2 mother-to-child cases

目的对山东省的2对均为人类免疫缺陷病毒Ⅰ型抗体阳性的母子感染毒株进行核酸检测和序列分析,以探讨他们感染毒株的起源关系。方法采集2对母子的外周静脉防凝血,提取前病毒DNA进行体外扩增,获得核心蛋白(gag)基因的核酸片段,并对其进行测定和分析。结果2对母子核酸扩增均为阳性,序列分析显示毒株亚型均为A/E,母子之间有高度同源性。结论由分子生物学研究和流行病学资料证实这2对母子是HIV-1母婴传播病例。

Objective To study the origin of HIV-1 strains from 2 mother-to child cases. Method DNA fragments of HIV 1 gag gene were amplified by nested PCR from the 4 target per sons, and the amplified region were sequenced. Results The sequence of the specified fragment from all persons were analyzed and the strains were belonged to subtype A/E, while the sequence between mother＇s and child＇s is highly homologous. Conclusion 2 children were infected by mother-to-child transmission.