摘要
目的了解广州某部新兵连戊型病毒性肝炎（戊型肝炎）暴发流行的分子病毒学特征,并与当地散发毒株比较,以查找病原可能来源.方法用逆转录-聚合酶链反应（RT-PCR）法,对抗HEV-IgM阳性的34例暴发性戊型肝炎及46例散发性戊型肝炎患者的血清和粪便标本进行HEV RNA检测,并对HEV RNA阳性标本的基因开放读码框（ORF）2部分片段进行克隆测序分析.结果 34例暴发流行病例标本中检测出12株病毒,46例散发病例标本中检到2株.经克隆测序分析,各暴发毒株的核苷酸同源性为95.3%～100%;氨基酸同源性为94.0%～100%.且暴发毒株和散发毒株的核苷酸及氨基酸的同源性也较高,分别为95.3%～99.3%和94.0%～100%;暴发毒株和散发毒株与各型中的标准株相比,与Japl株同源性最高,其核苷酸同源性为92.0%～95.3%,氨基酸同源性为96.0%～100.0%.进化树分析提示本次戊型肝炎暴发流行病毒株与戊型肝炎病毒基因Ⅳ型距离最近.结论 本次戊型肝炎暴发流行的病原可能来于广州本地;广州地区戊型肝炎流行毒株属戊型肝炎基因型Ⅳ型.
Objective To investigate the genotype and gene mutation of hepatitis E virus isolated from the serum and stool samples of the patients with epidemic outbreak hepatitis E in certain recruit barracks of Guangzhou. Methods The reverse transcription polymerase chain reaction （RT-PCR） was performed to amplify the partial ORF2 nucleotide acid sequences of hepatitis E virus isolated from 34 and 46 inpatients with epidemic outbreak and sporadic hepatitis E respectively. The PCR products of the positive samples were cloned and sequenced. Results The 14 strains, including 12 epidemic strains and 2 sporadic strains, were isolated from the total 80 inpatients. The homology of nucleotide acid and amino acid sequences of 12 epidemic outbreak strains is 95.3%- 100% and 94.0%-100% . The homology between epidemic outbreak strains and sporadic strains is 95.3%- 99.3% and 94.0%- 100 %. Compared with the standard different genotypes of H EV, these strains have the highest homology to the Japl strain which belongs to genotype IV, with homology of 96.0 %- 100%, respectively. Phylogenetic analysis indicated that these the nucleotide acid sequence homology of 92.0 %- 95.3% and amino acid sequence stains and Japl strain share the same cluster. Conclusion Epidemic outbreak strains isolated from the patients in recruit barracks of Guangzhou belong to the genotype of HEV and the nucleotide acid sequences had partial mutation.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2006年第2期109-112,共4页
Chinese Journal of Infectious Diseases
