摘要
建立了高效液相色谱法测定5-羟基色氨酸. 在优化条件下, 色谱柱： Symmetry C18柱;流动相： V（甲醇）：V（水）＝5：95;检测波长： 276 nm. 5-羟基色氨酸对照品溶液在0.8～4.0 μg的范围内与峰面积呈良好线性关系, r=0.9992;回收率为99.94%, RSD=1.35%.
A high performance liquid chromatography （HPLC） method was established for determination of 5- Hydroxytrytophan. The optimal experiment condition was using C18 column （Symmetry C18, 4.6 mm × 150 mm, 5μm）, mobile phase of methanol-water （5：95） and detection wavelength of 276 nm. The calibration curve was linear in the range of 0.8 - 4.0 μg for 5 HTP （ r = 0. 9992）. The average recovery （ n = 5 ） was 99.94 % for 5 HTP （ RSD = 1.35%）.
出处
《分析试验室》
CAS
CSCD
北大核心
2006年第5期59-61,共3页
Chinese Journal of Analysis Laboratory
