摘要
应用增殖细胞核抗原（PCNA）和C－myc单克隆抗体，以免疫组织化学方法检测正常膀胱组织和膀胱癌组织中Cmyc蛋白和PCNA的表达水平。结果表明：5例正常膀胱组织中未发现C－myc和PCNA阳性表达，阳性表达的C－myc和PCNA均定位于肿瘤的细胞核内。58例膀胱癌中C－myc和PCNA蛋白阳性表达分别为43．1％和62．1％，并且C－myc和PCNA阳性表达率与膀胱癌的病理分级、临床分期和患者术后生存率相关。提示C－myc和PCNA阳性表达与膀胱癌发生发展相关，并有可能成为评价膀胱癌预后的指标之一。
We examined the expression of PCNA and Cmyc in normal bladder tissues and bladder cancer ti-ssues by immunohistochemical method of C-myc and PCNA. The positive expression rates of C-myc and PCNA for bladder cancer were 43.1% and 62.1% respectively. Negative staining was found in 5 normal bladder tissues. Expression of C-myc and PCNA protein were related to the stage, grade and survival rate of bladder cancer, Our study suggests that expression of G-myc and PCNA may related to the development of bladder cancer. It is possible to be a marker of predicting the prognosis of bladder cancer.
