抑肽酶对人脐静脉内皮细胞活化的影响

Effects of aprotinin on the human umbilicus vein endothelial cells activation in vitro

目的体外观察抑肽酶对人脐静脉内皮细胞活化的影响,探讨其抑制体外循环术后系统性炎性反应的机制。方法将体外培养的人脐静脉内皮细胞随机分为空白组,人TNFα(肿瘤坏死因子α)活化组,抑肽酶+TNFα组,采用免疫细胞化学法观察人脐静脉内皮细胞细胞间黏附分子1(ICAM1)的表达。用细胞培养小室测定中性粒细胞跨迁单层融合的人脐静脉内皮细胞的迁移率。结果抑肽酶可显著抑制TNFα诱导的人脐静脉内皮细胞ICAM1的表达(P<0.05),抑肽酶对中性粒细胞跨迁活化的单层人脐静脉内皮细胞有显著影响(P<0.05)。结论抑肽酶可抑制TNFα诱导的人脐静脉内皮细胞的活化;抑肽酶可抑制中性粒细胞跨迁人TNFα活化的单层人脐静脉内皮细胞,抑肽酶可能通过抑制内皮细胞的活化来抑制体外循环术后系统性炎性反应。

Objective To observe the effecte on aprotinin of the human umbilicus vein endothelial cells activation in vltro,and to investigate the antl-inflammatory mechanism of aprotinin in the prevention of systemic inflammation after cardiopuulmonary bypass.Methods Human umbilicus vein endothelial cells（ECV-304） was cultured and randomly divided into three groups：control group,TNFα activating group, aprotinin ＋ TNFα group. Immunoeytochenuical staining was used to observe the changes in the expression of ICAM-1 ; we studied polymorphonuclear neutrophils transmigrating through cultured human umbilicus vein endothelial cells monohyers in response to the chemoattractants： plate.let-actlvating factor（PAF） by Using cell culture inserts.Resulls Aprotinin could downgrade the expression of ICAM-1 of TNFα activating endothelial cells; aprotinin had significant effect on neutrophils transmigration through cultured human umbilicus vein endothelial cells （ECV-304） monolayers in responsc to the chemoattractants： phtelet-activafing factor（ P 〈 0.05）. Conclusins Aprotinin can inhibit human umbilicus vein endothelial cells activation,and neutrophih trasmigration through cultured human umbilicus vein endothelial cells monolayers in response to the chemoattractants： platelet-activating factor. The anti-inflammatory mechanism of action of aprotinin during cardiopulmonary bypass may be completed by inhibiting endothelial cells activation.