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两种快速鉴定红色毛癣菌方法比较 被引量:1

Two Methods of Identification for Trichophyton rubrum
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摘要 目的对比改良Kane/Fischer皮肤癣菌的鉴定系统和特异性引物鉴定红色毛癣菌。方法利用改良Kane/Fischer皮肤癣菌的鉴定系统和特异性引物TR1F和TR1R,分别对32株皮肤癣菌进行培养和PCR扩增。结果改良Kane/Fischer皮肤癣菌的鉴定系统和特异性引物可以快速准确鉴定红色毛癣菌,但后者更快速、准确。结论这两种方法可用来快速、准确、敏感地鉴定红色毛癣菌。 OBJECTIVE To compare modified Kane/Fischer system and PCR method in identification for Trichophyton rubrum. METHODS Using modified Kane/Fischer system and TR1F and TR1R primers to identify 32 strains of dermatophytes. RESULTS The modified Kane/Fischer system and PCR could rapidly and stably identify T. rubrum. CONCLUSIONS The two methods can be used as the standard system to identify T. rubrum.
出处 《中华医院感染学杂志》 CAS CSCD 北大核心 2006年第5期592-594,共3页 Chinese Journal of Nosocomiology
基金 "十五"军队科研基金资助(01MA121)
关键词 Kane/Fischer系统 红色毛癣菌 PCR Kane/Fischer system Trichophyton rubrum PCR
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参考文献4

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同被引文献8

  • 1刘素玲,冉玉平,曾蔚,张浩,代亚玲,李明远,贾文祥.一种适用于PCR反应的酵母菌、无绿藻及丝状真菌DNA提取方法[J].中国真菌学杂志,2006,1(6):340-342. 被引量:28
  • 2钟志红,李若瑜,李冬梅,王端礼.红色毛癣菌随机扩增DNA多态性分型研究[J].中华医学检验杂志,1997,20(1):35-37. 被引量:9
  • 3Liu D,Pearce L,Lilley G,et al.PCR identification of dermatophyte fungi Trichophyton rubrum,T.soudanense and T.gourvilii[J].Med Microbiol,2002,51(2):117-122.
  • 4Jackson CJ,Barton RC,Kelly SL,et al.Strain identification of Trichophyton rubrum by specific amplification of subrepeat elements in the ribosomal DNA nontranscribed spacer[J].J Clin Microbiol,2000,38(12):4527-4534.
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  • 7Zaias N,Tosti A,Rebell G,et al.Autosomal dominant pattern of distal subungual onychomycosis caused by Trichophyton rubrum[J].J Am Aced Dermatol,1996,34 (2):302.
  • 8青春,冉玉平,李发增,代亚玲,赵国庆,刘素玲,何晓丹,周光平.多发性红色毛癣菌病患者不同部位菌株的分子鉴定[J].中华皮肤科杂志,2008,41(8):552-553. 被引量:4

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