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FGF18对成牙本质细胞样细胞增殖和碱性磷酸酶活性的影响 被引量：5

Effect of FGF18 on proliferation and ALPase activity of odontoblastoid cell
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摘要 目的:研究FGF18对成牙本质细胞样细胞MDPC-23增殖和ALPase活性的影响。方法:用MTT法和酶动力法分别检测MDPC-23细胞经不同浓度的FGF18刺激48 h和72 h后细胞增殖和ALPase活性的变化。结果:在一定范围内,FGF18显著促进MDPC-23细胞的增殖和ALPase活性,随着浓度的增高和时间的延长,作用更加明显。结论:FGF18可能促进小鼠成牙本质细胞的增殖和分化。 Objective：To investigate the effect of FGF18 on proliferation and ALPase activity of odontoblastoid cell line MDPC-23. Method：After incubation with FGF18 of different concentration for 48 or 72 h, the proliferation and AL- Pase activity of MDPC-23 cell were examined and analyzed. Result：The proliferation and ALPase activity were facilitated by exogenous FGF18 and the facilitation is dependent on FGF18 concentration and incubating time. Conclusion： FGF18 may play a role during the development of dental germ and could improve the proliferation and differentiation of odontoblast.
作者 姜永 杨帆 肖明振 何文喜 范晓敏
机构地区 第四军医大学口腔医院牙体科
出处 《临床口腔医学杂志》 2006年第5期269-271,共3页 Journal of Clinical Stomatology
基金 国家自然科学基金资助项目(30300385)
关键词 FGF18 MDPC-23 细胞增殖 ALPase活性 FGF18 MDPC-23 proliferation ALPase activity
分类号 R780.1 [医药卫生—口腔医学]
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参考文献8

  • 1Hanks CT,Fang D,Sun Z,et al.Dentin-specific proteins in MDPC-23 cell line[J].Eur J Oral Sci,1998,106(1):260-266.
  • 2Ruch JV,Lesot H,Begue-Kirn C.Odontoblast differentiation[J].Int J Dev Biol,1995,39(1):51.
  • 3Quarles LD,Yohay DA,Lever LW,et al.Distinct proliferative and differentiated stages of murine MC3T3-E1 cells in culture:an in vitro model of osteoblast development[J].J Bone Miner Res,1992,7(6):683-687.
  • 4Thonemann B,Schmalz G.Immortalization of bovine dental papilla cells with simian virus 40 large T antigen[J].Arch Oral Biol,2000,45(10):857-869.
  • 5Usui H,Shibayama M,Ohbayashi N,et al.Fgf18 is required for embryonic lung alveolar development[J].Biochem Biophys Res Commun,2004,322(3):887-892.
  • 6Reinhold MI,Abe M,Kapadia RM,et al.FGF18 represses noggin expression and is induced by calcineurin[J].J Biol Chem,2004,279(37):38209-19.
  • 7Ellsworth JL,Berry J,Bukowski T.Fibroblast growth factor-18 is a trophic factor for mature chondrocytes and their progenitors[J].Osteoarthritis Cartilage,2002,10(4):308-320.
  • 8杨帆,肖明振,赵守亮.小鼠牙胚fgf18的基因克隆和序列分析[J].临床口腔医学杂志,2003,19(4):200-202. 被引量：5

二级参考文献5

  • 1[1]Whitmore TE, Maurer MF, Sexson S, et al.Assignment of fibroblast growth factor 18 (fgf18) to human chromosome 5q34 by use of radiation hybrid mapping and fluorescence in situ hybridization[J]. Cytogenet Cell Genet, 2000,90:231～3.
  • 2[2]Ohuchi H, Kimura S, Watamoto M, et al.Involvement of fibroblast growth factor fgf 18-fgf8 signaling in specification of left-right asymmetry and brain and limb development of the chick embryo[J]. Mech Dev, 2000,95:55～66.
  • 3[3]Ellsworth JL, Berry J, Bukowski T, et al.Fibroblast growth factor-18 is a trophic factor for mature chondrocytes and their progenitors[J]. Osteoarthritis Cartilage, 2002,10:308～20.
  • 4[4]Liu Z, Xu J, Colvin JS,et al.Coordination of chondrogenesis and osteogenesis by fibroblast growth factor 18[J]. Genes Dev ,2002 ,16:859～69.
  • 5[5]Ohbayashi N, Shibayama M, Kurotaki Y.fgf18 is required for normal cell proliferation and differentiation during osteogenesis and chondrogenesis[J]. Genes ev ,2002 16:870～9.

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临床口腔医学杂志
2006年 第5期

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