IBH中法氏囊B淋巴细胞消长规律的研究

Study on Transform Regulation of B Lymphocytes of IBH in Bursal

将180只1日龄SPF雏鸡随机分成对照组和试验组(攻毒组),各90只,对试验组鸡进行口服接种鸡腺病毒Ⅷ型毒株(1∶50倍)0.3 mL/只。两组鸡分别在攻毒后第1,3,5,7,9,12,15,20天和25天,心脏采血致死,取其法氏囊,分离其淋巴细胞并制成悬浮液,在流式细胞仪上用双染色法检测法氏囊中Bu-1+B淋巴细胞,探讨鸡包涵体肝炎的发病过程中,机体受病原刺激后B淋巴细胞在法氏囊中的消长规律。结果表明,在整个病程中法氏囊一直为脾脏供给大量的成熟B淋巴细胞,且B淋巴细胞活性显著增强,在7 d时Pi(post infection)达到最高峰。

180 one-day-old SPF chickens were divided into two groups randomly. Control and infectious groups with 90 birds,respectively, were inoculated chicken gland virus Ⅷ （1：50 time＇s dilution） orally 0.3ml per chicken, and killed by getting blood sampling from heart respectively at 1,3,5,7,9,12,15,20 and 25 days postinfection. The blood, spleen and Bursal were collected and made into the lymphocyte suspension, and the double staining method was used to examine the Bu-1＋ B lymphocytes in Bursal by flow cytometer and define the growth and decay regulation of B lymphocytes in Bursal after the stimulation of antigen in the process of inclusion body hepatitis. The results demonstrated that the Bursal always provided numerous adult B lymphocytes to spleen and blood, and the activity of B lymphocytes strengthen notably, and achieved the summit at 7 days.