补肾益气化瘀方对亚急性衰老大鼠学习记忆功能及海马酶学的影响

Effects of Bushen-Yiqi-Huayu prescription on learning-memory ability and hippocampal enzymology in the subacut aging rats

目的观察补肾益气化瘀方对亚急性衰老大鼠学习记忆功能的改善作用,并探讨其作用机理。方法用腹腔注射D-半乳糖连续6周,建立亚急性衰老大鼠模型。模型建立后,药物组每天分别用补肾益气化瘀方提取液灌胃,空白对照组、模型对照组用生理盐水灌胃,连续4周。然后用morris水迷宫实验测试大鼠学习记忆功能,紫外分光光度计检测大鼠海马丙二醛(MDA)含量及超氧化物歧化酶(SOD)、乙酰胆碱酯酶(AchE)酶学活性。结果药物高剂量组与造模组相比:大鼠水迷宫实验平均潜伏期明显缩短[(11.33±3.73)svs(32.25±9.57)s](P<0.01);大鼠海马MDA含量减少,分别为(3.50±1.06)nmol/mgprot和(4.83±0.97)nmol/mgprot(P<0.01);AchE的活性降低,分别为(0.36±0.07)U/mgprot和(0.50±0.04)U/mgprot(P<0.01);SOD活性增加,分别为(522.11±68.62)U/mgprot和(404.61±66.90)U/mg-prot(P<0.01)。结论补肾益气化瘀方对亚急性衰老大鼠学习记忆功能具有促进作用,作用机理可能与其具有抗自由基作用及抑制AchE的活性有关。

Objective To observe the role of Bushcn-Yiqi-Huayu prescription＇s amelioration of learningmemory ability in subacut aging rats and explore the mechanism of it. Methods The model of subacut aging rats were set up by injection D-galactose into abdominal cavity for 6 weeks. After model set up,drug groups were administrated with extrated fluid of Bushen-Yiqi-Huayu prescription for 4 weeks, blank and model groups were administrated with saline. The learning-memory ability of rats were tested by morris water maze test, the content of MDA and enzymology activity of SOD and AchE in hippocampi of rats were detected by ultraviolet spectrophotometer. Resuits To contrast high drug group with model group, tile rats had less time in averagely latent period of water maze test（ P〈0. 01 ） ,had less content of MDA and activity of AchE ,but more activity of SOD in hippocampi. Conclusion Bushen-Yiqi-Huayu prescription may promote the learning-memory ability of subacut aging rats, and the mechanism is related to the role of its anti-free radical and reducing the activity of AchE .