芦笋在PEG模拟干旱条件下的生理生化变化

Study on biophysical-chemical change of asparagus under PEG stress

用聚乙二醇(PEG-6000)胁迫处理芦笋幼苗,检测了幼苗的渗透调解物质含量、生物膜透性、抗氧化特性等指标。结果表明:脯氨酸、可溶性糖含量随着处理时间的延长显著增加,且以20%的PEG浓度处理增加最明显;SOD、POD、CAT活性在PEG处理后明显增强;相对电导率随着处理浓度的增大和时间的延长而增大;MDA含量随着处理时间的延长明显增加,且以10%和20%浓度处理增加显著。由此表明,芦笋在干旱胁迫下通过增加渗透调解物质含量,降低水势来提高其抗旱能力;通过增强抗氧化酶活性,提高抗氧化能力,来减轻干旱胁迫伤害。

Investigation was carried out on osmotic permeability of biomembrane and antioxidization characteristics of asparagus under PEG stress with the concentrations of 10%, 20% and 30%. The result showed that the contents of free Pro and soluble sucrose increased significantly with the test time, and reached the maximum at the treatment of 20% PEG-6000. The activities of CAT, POD and SOD increased with the concentration of PEG-6000, moreover, the relative conductivity and MDA increased with the test time and the concentrations of PEG-6000. This indicated that Asparagus officinlis seedlings adapted to drought stress by adjusting osmotic substance, decreasing water potential and improving protective enzymes activity to improve antioxydization ability under drought stress.