复方薤白胶囊对野百合碱诱导的肺动脉高压大鼠病理的影响

Effect of Compound Bulbus Allii Macrostemi Capsule on Pathology of Monocrotaline-induced Pulmonary Artery Hypertension in Rats

目的探讨复方薤白胶囊(FFXBC)对野百合碱(Monocrotaline MCT)诱导的肺动脉高压(PH)大鼠肺小动脉、右心室、肺组织病理的影响。方法SD(Sprague-Dawley)大鼠84只,随机分为7组:正常组10只、模型组14只、桂龙咳喘宁组、地塞米松组、卡托普利组、FFXBC高剂量组、FFXBC低剂量组各12只。MCT进行1次性腹腔注射(按60 mg/kg),正常对照组一次性腹腔注射等量的生理盐水。于造模d 2开始给药。给药d 21,每组随机取6只进行平均肺动脉压(mPAP)、平均右心室压(mRVP)测定,每组随机取8只大鼠进行观察肺小动脉、右心室及肺组织病理形态学变化。结果模型组与正常对照组相比,mPAP、mRVP明显增高,差别有明显统计学意义(P<0.01);模型组小动脉及右心室厚度明显增厚,肺小动脉管腔狭窄甚至闭塞,肺组织炎性细胞浸润非常明显。桂龙咳喘宁组、地塞米松组、卡托普利组、FFXBC高剂量组、FFXBC低剂量组与模型组相比,mPAP、mRVP明显下降,差别有统计学意义(P<0.05或P<0.01),肺小动脉及右心室壁增厚减轻;肺组织炎性细胞浸润减轻,组间比FFXBC高剂量组效果明显优于FFXBC低剂量组。结论FFXBC能有效的降低MCT诱导大鼠PH的作用,可能的机制是抑制、改善肺小动脉及右心室重构,减轻MCT引起的炎性细胞浸润现象,有效降低肺动脉压及右心室压。提示FFXBC将是防治PH的很有前途的中药,值得进一步的研究。

OBJECTIVE To investigate into the effect of Compound Bulbus Allii Macrostemi Capsule on the pathology of arteriole, right ventricle and lung tissue of rats with hypertension induced by monocrotaline （MCT）. METHOD 84 SD （Sprague - Dawley） rats were randomly divided into 7 groups： a normal group of 10 rats, a model group of 14 rats, a Guilong Kechuanning group of 12 rats, a methylprednisolone group of 12 rats, a captopril group of 12 rats, a Compound Bulbus Allii Macrostemi Capsule high dosage group of 12 rats and a Compound Bulbus Allii Macrostemi Capsule low dosage group of 12 rats. Peritoneal monocrotaline injection （60 mg/kg） was given. The normal control group was given saline injection of the same dosage. Twenty - one days after the injection, six rats were taken randomly from each group to receive determination of average pulmonary artery pressure （mPAP） and average right ventricle pressure （mRVP）. Eight rats was taken randomly from each group to observe pathological and morphological changes in the arteriole, the right ventricle and the lung tissue. RESULT Compared with the control group, mPAP and mRVP in the model group increased markedly, with marked statistical significance （P 〈 0.01 ）, arteriole and right ventricle thickened markedly, the cavity of the arteriole narrowed or even closed and there was marked infiltration of inflammatory cells of the lung tissue. In the Guilong Kechuanning group, the methylprednisolone group, the captopril group, the Compound Bulbus Allii Macrostemi Capsule high dosage group and the Compound Bulbus Allii Macrostemi Capsule low dosage group, mPAP and mRVP dropped markedly, with marked statistical significance （ P 〈 0.05 or P 〈 0.01）, arteriole and right ventricle thickened markedly, the cavity of the arteriole narrowed or even closed, and the infiltration of inflammatory cells of the lung tissue was relieved. CONCLUSION Compound Bulbus Allii Macrostemi Capsule can effectively relieve MCT - induced pulmonary hypertension in rats, the possible mechanism of which may lie in the inhibition and improvement of reconstruction of arteriole and right ventricle, the relief of inflammatory cell infiltration and the effective reduction of pulmonary pressure and right ventricle pressure. This study indicates that Compound Bulbus Allii Macrostemi Capsule is a promising recipe for the treatment and prevention of pulmonary hypertension and further study is indicated.