南江黄羊FSHβ亚基基因内含子1序列的测定

Sequencing and Analyzing of Follicular-stimulating Hormone Beta Subunit Gene In-1 from Nanjiang Huang Goat

利用牛、绵羊等动物FSHβ亚基基因序列设计引物,采用PCR法扩增并测定出南江黄羊FSHβ亚基基因内含子1全序列(GenBank登录号:AY838283)。山羊FSHβ基因内含子1全序列长641bp,A、C、G、T 4种碱基分别占35.73%,13.88%,17.47%,32.92%;A+T(68.65%)的含量远高于G+C(31.35%)的含量。所测定7个个体的碱基序列完全一致,表明FSHβ基因内含子1的保守性较强。

According to the FSH β gene sequence of bovine and sheep, the primers were designed and follicular-stimulating hormone （FSH） β gene in- 1 from Nanjiang Huang goat was sequenced. In the complete 641 base pairs of FSH β gene in- 1, the contents of A,C,G and T were 35.73%, 13.88%, 17.47 % and 32.92 %, respectively, the content of A ＋ T （68.65 % ） was higher than that of G ＋ C （31.35 % ）. The gene sequences from seven individuals were completely similar, which indicated that FSH β gene in- 1 was very conservative.