砷对仔代大鼠神经行为和学习记忆功能影响

Effects of arsenite on neurobehavior and learning and memory in offspring of rats

目的研究饮水砷暴露对大鼠仔鼠神经行为发育和学习记忆功能的影响。方法雌性大鼠于受孕后第6 d开始以自由饮水方式分别暴露于10,50和100 mg/L的NaAsO2水溶液,连续染毒直到仔鼠出生后第42 d。观察砷对仔代大鼠生理发育、神经行为发育及学习记忆能力的影响。结果100 mg/L砷剂量组仔鼠从出生后第12 d起身长、体重明显低于对照组,但张耳、开眼、长毛、出牙、抬头、爬行、站立和行走等生理发育指标与对照组相比差异无统计学意义。在神经行为测试中,100 mg/L砷剂量组仔鼠尾部悬挂、听觉惊愕和视觉定位等指标阳性率显著低于对照组,出现神经行为发育迟缓。方位水迷宫实验中,各砷暴露组仔鼠在记忆获得测试和记忆保留测试中能正确寻找隐蔽平台所需训练次数显著多于对照组,说明砷对仔鼠短时记忆和长时记忆均有一定影响。结论饮水砷暴露可对仔鼠神经行为和学习记忆产生毒性作用。

Objective To study effects on development and behavior, learning and memory in the offspring of rats induced by arsenite through drinking water. Methods Wister rats were exposed to arsenite（ 10, 50,100 mg/L, respectively in drinking water）from gestation day 6 until F1 pups 42 days old. F1 pups were examined to observe the impact of arsenite on the early physiological, neurobehavioral, learning and memory. Results The stature and weight of F1 pups in group of 100 mg/L iAs were lower than in control after postnatal day 12. But other physiologic markers（eye opening, pinna detachment, hair growth, tooth growth, et al） were not affected by iAs exposure. In contrast to the control group, visual placing, acoustic startle and tail suspending in group of 100 mg/Larsenits were delayed. In bearings water labyrinth tests, the training times of F1 pups in arsenite exposed groups swimming correctly to the hidden - platform in acquired memory test and memory reservation test were more than in control, which showed that learning and memory abilities of F1 pups were affected by arsenite exposure. Conclusion Neurobehavioral development and abilities of learning and memory in offspring rats could be impaired by arsenite exposure throught drinking water.