尼莫地平对戊四氮致癫痫大鼠的抗氧化作用

ANTIOXIDANT EFFECT OF NIMODIPINE ON RATS WITH EPILEPSY INDUCED BY PENTETRAZOLE

目的探讨尼莫地平对实验性癫痫鼠的抗氧化作用.方法 50只Wistar大鼠随机分为5组,各10只.正常对照组：腹腔注射生理盐水;癫痫组：腹腔注射戊四氮（PTZ）50 mg/kg, 诱发癫痫后30 min处死;尼莫地平0.1、0.3、0.5 mg/kg 组：腹腔注射PTZ 50 mg/kg前15 min分别注射尼莫地平注射液0.1、0.3、0.5 mg/kg, 每隔24 h重复1次,连续3 d后处死.观察各组大鼠癫痫样发作的潜伏期、持续时间、发作形式,并测定各组大鼠脑组织超氧化物歧化酶（SOD）、丙二醛（MDA）、谷光甘肽过氧化物酶（GSH-PX）活性.结果尼莫地平预处理各组大鼠癫痫发作类型以轻型发作为主,发作潜伏期明显延长,持续时间短; SOD活性较癫痫组明显增高,MDA、GSH-PX活性明显下降,差异均有显著性（F＝10.720～35.831,P〈0.01）.结论尼莫地平可通过抗氧化机制来发挥抗癫痫作用.

Objective To explore the antioxidant role of nimodipine in experimental-epilepsy rats. Methods Fifty Wistar rats were divided into 5 groups： control group, epilepsy group, and 0. 1 mg-, 0.3 mg-, and 0.5 mg-nimodipine groups. The incubation period, duration, and the onset of epilepsy were observed and SOD, MDA, and GSH PX of brain tissue were determined in the nirnodipine group. The results were compared with those of the PTZ epilepsy group. Results The onset in nimodipinepretreatment group was characterized by light seizure, prolonged incubation time, short duration and high SOD, low MDA and GSH-PX, There were significantly difference between them （F= 10. 720 35. 831,P〈0. 01）. Conclusion Antiepileptic role of nimodipine may be achieved by its antioxidation.