摘要
目的 探讨更具有优越性的腰椎间盘细胞培养方法.方法 16例胎儿及成人腰椎间盘细胞分别进行单层培养和立体培养,检测细胞分裂指数,进行对比分析.结果 立体培养细胞指数生长期分裂指数较单层培养细胞明显增高（t=5.49～9.61,P〈0.05）,细胞稳定生长期两者分裂指数无明显差异（P〉0.05）.结论 立体培养的细胞较单层培养者具有更大的生物学活性.
Objective To study a much better way to culture lumbar intervertebral disc cells. Methods The lumbar intervertebral disc cells of 16 fetuses and adults were cultured by monolayer and three dimension methods respectively. The cell division indexes were detected and compared. Results The cell division indexes of the three-dimension group were significantly higher than those of the monolayer group during the exponential growth phase （t=5.49-9.61, P〈0.05） ,but not during the stable growth phase （P〉0.05）. Conclusion Three-dimension cultured cells show more biological activity than those by monolayer culture.
出处
《齐鲁医学杂志》
2006年第3期221-222,共2页
Medical Journal of Qilu
基金
国家自然科学基金资助项目(C30303064)
关键词
椎间盘
细胞培养技术
细胞分裂
intervertebral disc
cell culture technique
cell division
