流体剪切力作用下鼠极化破骨细胞的形态变化

Effect of Fluid Shear Stress Time on the Morphological Change of Rat Polarized Osteoclasts

目的探讨流体剪切力作用下大鼠极化破骨细胞的形态变化。方法机械分离培养大鼠极化破骨细胞，对破骨细胞鉴定后，采用本课题组白行研制的流体剪切力装置在0．29Pa条件下，观察流体剪切力作用0、15、30、45、60、75、90、105、120min时破骨细胞的形态变化。结果大鼠极化破骨细胞抗泊石酸酸性磷酸酶（TRAP）染色阳性，吸收试验阳性，细胞形态较大，直径约30μm，形状不规则以类圆形多见，核3～20个，可见空泡，周边大量细胞丝。随流体剪切力作用时间的延长，极化破骨细胞面积、直径均有增大趋势，30min组、105min组与0min组（对照组）间差异有统计学意义（P〈0．05）。结论在本试验条件下，随流体剪切力作用时间的增加，SD-大鼠极化破骨细胞的形态呈波动性变化，直径和面积均有增大的趋势。

Objective To elucidate the morphological change of rat polarized osteoclasts influenced by different fluid shear stress time. Methods Special TRAP dyeing and bone resorption of rat polarized osteoclasts were performed. A fluid shear stress device made in the authors＇ laboratory was used to enforce shear stress at different times on the polarized osteoclasts for observation on their morphological change and analysis on the kind of change by image analytical software. The time points were 0,15,30,45,60,75,90,105,120 min; the shear stress was 0. 29 Pa. Results Morphologically, the polarized osteoclasts were bigger than other monocytes. They were each about 30 μm in diameter, having about 20 round and black nuclei in cytoplasm, There were large numbers of cellular microfilaments. The areas and diameters of these osteoclasts increased with the times. Statistically, oneway ANOVA showed significant differences between the 0 min group and 30 min, 105 min groups respectively, Conclusion In this study, the morphological change of polarized osteoclasts fluctuated with the increase of fluid shear stress time, and their areas and diameters exhibited a tendency to increase.