实时荧光定量PCR检测核糖体蛋白S13基因在NK/T细胞淋巴瘤中的表达

Real-time Fluorescence Quantitative PCR in Detecting Ribosome Protein S13(RPS13) Gene Expression in NK/T Cell Lymphoma

目的应用实时荧光定量PCR技术检测NK/T细胞淋巴瘤核糖体蛋白S13(RPS13)基因的表达。方法提取石蜡包埋NK/T细胞淋巴瘤组织总RNA,逆转录为cDNA。应用实时荧光定量PCR技术检测NK/T细胞淋巴瘤RPS13表达水平。结果20例NK/T细胞淋巴瘤RPS13表达水平明显低于对照。结论RPS13基因表达水平明显低于正常人外周血淋巴细胞,这可能在NK/T细胞淋巴瘤发生、发展中起到一定作用。

Objective To quantitatively detect the expression level of ribosome protein S13 （RPS13） in NK/T cell lymphoma. Methods The total RNA isolated from the parafin-embedded tissue of NK/T cell lymphoma was reversely transcribed into cDNA. The real-time fluorescence quantitative PCR technology method was used to analyze the expression level of RPS13 gene. Results The real-time fluorescence quantitative PCR technology was successfully performed to precisely detect the mRNA level. The expression level of RPS13 gene in tumor tissue of 20 cases of NK/T cell lymphoma was dramatically lower than that in normal peripheral blood lymphocyte of healthy controls. Conclusion The RPS13 gene has lower expression level in tumor tissue cells of NK/T cell lymphoma than in normal lymphocyte, indicating that it plays an role in the development of the NK/T cell lymphoma.