保健食品中甘露醇的毛细管气相色谱分析

Determination of Mannitol in Health Food by Capillary Gas Chromatography

目的建立保健食品中甘露醇的毛细管气相色谱分析方法。方法用去离子水超声波提取样品中的甘露醇，经乙酸酐衍生化为沸点较低的甘露醇六乙酯，用SPB-608毛细管色谱柱分离，火焰离子化检测器（FID）进行检测。色谱柱温度为180℃、进样口和检测器的温度均为210℃，氮气流速30mL／min。结果优化了样品衍生化和色谱分析条件，实现了葡萄糖、肌醇和山梨醇与甘露醇的分离，排除了它们对甘露醇测定的干扰。本方法的线性范围为（0．05～10．00）mg／mL，检出限为0．01mg／mL，日内精密度RSD为4．4％，日间精密度RSD为7．3％～7．60%，平均加标回收率为84．6%～107．6%。结论本方法操作简单、灵敏、可靠，能满足保健食品中甘露醇定量分析的要求。

Objective To develop a capillary gas chromatographic method for the determination of mannitol in health food. Methods Mannitol was extracted from the sample with water by ultrasonic wave. The aliquot of sample solution was acetylated to form mannitol hexacetate with lower boiling point. Then the derivative was separated with the use of SPB-608 capillary chromatographic column and determined by flame ionization detector （FID） at the column temperature of 180 ℃, the detector temperature of 210 ℃ and the nitrogen flow rate of 30 mL/min. Results The linear range of this method was 0. 05-10. 00 mg/mL and the limit of detection of was 0. 01 mg/mL. The within-day and between-days precisions were 4. 4% and 7. 3%-7. 6%, respectively. The average recoveries were 84. 6%-107. 6%. Mannitol could be separated from glucose, inositol and sorbitol under the optimum chromatographic condition. Conclusion The results demonstrate that the method has the advantages of simplicity, accuracy and sensitivity. It is suitable for the determination of mannitol in health food.