急性淋巴细胞白血病患者GSTπ、P170表达及临床意义

Expressinon and clinical significances of GSTπ and P170 in acute lypmphoblastic leukemia

目的检测急性淋巴细胞白血病(ALL)患者白血病细胞中谷胱甘肽-S-转移酶π(GSTπ)及P170的表达,探讨其与ALL患者临床特征的关系。方法应用免疫细胞化学(ABC)法检测43例ALL患者白血病细胞中GSTπ及P170蛋白表达状况。结果ALL患者GSTπ的阳性率32·5%。复发组ALL患者GSTπ表达显著高于初发ALL(47·4%vs20·8%,P<0·05);GSTπ阳性组ALL患者完全缓解(CR)率显著低于GSTπ阴性组(7·1%vs70·0%,P<0·01)。GSTπ与P170双阳性组CR率显著低于单纯P170/GSTπ阳性患者、GSTπ和P170双阴性组(P<0·001)。结论ALL患者中GSTπ表达可能与患者对化疗耐药及疗效密切相关;GSTπ与P170双阳性可能对化疗疗效和预后判断更有参考价值。

Objective ：To detect the expression of glutathione-s-transferase π （GSTπ） and P170 in acute lymphoblastic leukenia（ ALL）, and evaluate the relationship between GSTπ and clinical characteristics of ALL. Methods： Immunocytocbemical ABC assay was adopted to detect GSTπ protein in the cells. Results：The expression of GSTπ and P170 in relapsed ALL was higher than that in newly-diagnosed ALL（ P 〈0. 05 ）. In the group with GSTπ positive patients, the CR rate was lower compared to that in the GSTπ negative group（P 〈0. 05）, Moreover, the CR rate in patients with both GSTπ and P170 positive was lower than that in P170 positive group （ P 〈 0. 05 ） or in patients with beth GSTπ and P170 （ P 〈 0. 005 ）. Conclusion： GSTπ expression in ALL was probably associated with the efficicy and acquired chemoresistance. The co-expression of GSTπ and PI70 could predict chemoresistance more precisely.