AFLP标记在玉米种质资源鉴定中的应用

Identification of Corn Germplasms by AFLP Markers

利用AFLP分子标记技术对55份玉米(Z ea m ay s L.)种质材料进行了研究.结果表明,20对AFLP引物检测到清晰可见的带656条,其中多态性带487条.平均每个引物组合可以检测到24.4条多态性带.将55份玉米材料聚为6组,与材料来源基本一致.结果显示AFLP有利于玉米遗传多样性分析.对来源美国先锋的P ioneer系列与瑞得种群和BSSS种群的关系进行了初步探讨,认为P ioneer系列中有很多材料与瑞得种群有亲缘关系.

AFLP was adopted to study 55 germplasm materials, 656 bands were detected with 20 pairs of AFLP primers and 487 of them were polymorphic. On average eachprimer combination could be used to detect 24.4 polymorphic bands. The 55 materials were clustered into 6 groups,which were identical to the groups divided by their origins. These results showed that AFLP was conducive to analyzing the genetic diversity of corn. Further analysis of the relation of the Pioneer population of American origin to Reid and BSSS populations indicated that many materials of Pioneer populations had genetic relations to the Reid populations.