尿多酸肽对乳腺癌细胞周期的影响

Effect of Uroacitides on Cell Cycle Progression of Breast Cancer Cells

目的研究肿瘤细胞分化诱导剂尿多酸肽(CDAⅡ)对乳腺癌细胞周期的影响。方法将CDAⅡ与乳腺癌细胞株MCF7和MDA MB231进行体外培养,同时以维甲酸为阳性对照,观察其对乳腺癌细胞生长曲线、细胞周期、形态学等方面的影响。结果CDAⅡ可减缓两株乳腺癌细胞的生长和增殖能力,使乳腺癌细胞的细胞周期的分布出现S期比例减少,G0/G1期增加。结论CDAⅡ可抑制不同生物学特性的两株乳腺癌细胞的生长和增殖能力,细胞周期出现G0/G1期阻滞,为CDAⅡ治疗乳腺癌提供了实验依据。

Objective To investigate the effect of CDA-Ⅱ on the cell cycle progression of breast cancer cells. Methods The effects of CDA-Ⅱ on growth curve, cell cycle progression and morphology of breast cancer cell lines MCF-7 and MDA-MB-231 were observed when CDA-Ⅱ and MCF-7 or CDA-Ⅱ and MDA-MB-231 were blended to cultivate in vitro, in comparison with the classical cell differentiation inducer ATRA. Results CDA-Ⅱ decreased the growth speed and inhibit proliferation ability in breast cancer cell lines MCF-7 and MDA-MB-231. It caused G0/ G1 phase block of cell cycle and reduced the rate of S phase of breast cancer ceils. Conclusion CDA-Ⅱ has remarkable effect of anti-cell-proliferation and can induce cell cycle block of G0/G1 on breast cancer cells. This results provide experimental bases for the treatment of breast cancer with CDA-Ⅱ.