细菌性腹膜炎诱导大鼠多器官功能不全综合征后结肠运动的变化和机理

Changes and Mechanism of Colon Motility of the Rats in Multiple Organ Dysfunction Syndrome Induced by Bacterial Peritonitis

目的探讨细菌性腹膜炎致多器官功能不全综合征(MODS)大鼠结肠运动的变化和细胞因子白介素6(IL6)、肿瘤坏死因子α(TNF-α)及诱生性一氧化氮合成酶(iNOS)对结肠运动的影响。方法将Wistar大鼠随机分成正常对照组和MODS组;分别观测2组大鼠排便的粪点数、结肠肌条的振幅、结肠平滑肌细胞长度以及血清一氧化氮(NO)的变化;利用免疫组化和逆转录聚合酶链反应(RT PCR)方法检测结肠平滑肌IL-6、TNF-α和iNOS表达的变化。结果MODS组排便的粪点数和肌条的振幅均明显低于正常对照组(P<0.05);IL-6、TNF-α和iNOS蛋白表达在正常对照组为阴性表达,在MODS组为阳性表达;在正常对照组未检出IL6mRNA、TNF-αmRNA和iNOSmRNA的表达,而在MODS组,IL6mRNA、TNFαmRNA和iNOSmRNA均呈阳性表达;MODS组平滑肌细胞的长度和血清NO水平均较正常对照组明显增高(P<0.01)。结论MODS后结肠运动功能障碍与iNOS、IL6和TNF-α密切相关。

Objective To explore the changes of colon motility of the rats in multiple organ dysfunction syndrome （MODS） induced bacterial peritonitis and the effects of IL-6, TNF-α and induce nitricoxide synthase （iNOS） on colon motility. Methods Wistar rats were divided into two groups, which were the control group and the MODS group. The number of stool, the amplitude changes of circular smooth muscle strip, the length of smooth muscle cell, and the changes of serum NO in two groups were observed. The expressions of IL-6, TNF-α and iNOS protein and IL-6 mRNA, TNF-α mRNA and iNOS mRNA in distal colon were investigated by using immunohistochemical methods and RT-PCR. Results The numbers of stool and the amplitude in the MODS group were lower than those of the control group （P〈0.05）. The expressions of IL-6, TNF-α and iNOS were negative in the control group, while they were positive in the MODS group. IL-6 mRNA,TNF-α mRNA and iNOS mRNA were negative expression in the control group, but they were positive expression in the MODS group. The concentration of serum NO and the length of smooth muscle cells in the MODS group were higher than those of the control group （P〈0.01）. Conclusion Colon motor dysfunction of the rats is related to the iNOS, IL-6 and TNF-α.