胆囊胆固醇结石与肝细胞胆管膜上转运子BSEP、MRP2和MDR3关系的研究

Study about the Relationships Between the Formation of Cholesterol Calculus and the Transporters of BSEP, MRP2 and MDR3

目的探讨肝细胞胆管膜上转运子BSEP、MRP2和MDR3与胆囊胆固醇结石形成的关系。方法收集胆囊胆固醇结石患者的肝组织标本20例,正常肝组织标本10例。应用逆转录聚合酶链反应(RT PCR)技术和Westernblot技术检测BSEP、MRP2及MDR3mRNA和蛋白的表达。结果胆囊胆固醇结石患者肝组织中BSEP、MRP2和MDR3的mRNA及蛋白表达均显著低于正常肝组织(P<0.01)。结论BSEP、MRP2和MDR3的表达降低可能与胆囊胆固醇结石的形成有关。

Objective To evaluate the relationships between the transporters BSEP, MRP2, MDR3 and cholesterol calculus formation. Methods Twenty hepatic tissue specimens were taken from consented patients with cholesterol calculus during intraoperative liver biopsy, of which ten were taken from patients without cholesterol calculus. RNA of liver tissue from all the samples was extracted and ultraviolet spectrophotometry was used to measure the content and purity of it. The mRNA and protein expressions of BSEP, MRP2 and MDR3 were determined by reverse transcription-polymerase chain reaction （RT PCR） and Western blot analysis, respectively. Results RT-PCR showed that the mRNA expressions of BSEP, MRP2 and MDR3 in liver were significantly lower in patients with cholesterol calculus （0. 47±0.18, 1. 12±0.39 and 1. 02±0.24） than those in the liver of patients without calculus （0.90±0.42, 2.48±0.89 and 1. 94±0.80）,P〈0.01. And Western blot also showed the protein expressions of these transporters were significantly lower in patients with cholesterol calculus （90. 16± 18.82, 45.43± 22.77 and 61. 08±14.77） than those in the liver of patients without calculus （186. 17±4.34, 160.47±30. 19 and 100. 84± 15.44）. Conclusion The decreased expression of BSEP, MRP2 and MDR3 may correlate with the formation of cholesterol calculus.