原发性肝癌组织中芳香化酶和存活素的表达及其临床病理意义

Expression of Aromatase and survivin and Their Clinicopathologic Significance in Primary Hepatocarcinoma

目的研究芳香化酶(aromatase,Arom)和存活素(survivin,Surv)在原发性肝癌(primary hepatocarcinoma,PHC)中的表达情况,探讨它们间的相互关系以及与PHC临床病理特征之间的关系。方法应用ABC免疫组化染色法对47例PHC手术切除标本切片分别检测Arom和Surv的表达,并于高倍镜下评分。结果47例PHC癌组织中Arom和Surv的表达阳性率和表达评分均显著高于癌旁组织:Arom,40.43%vs21.38%(P<0.05),1.53±1.69vs0.79±1.41(P<0.05);Surv,63.83%vs31.91%(P<0.01),2.40±1.96vs1.45±1.80(P<0.05)。除Surv在肿块最大径<5cm的癌组织中的表达评分(4.00±2.10)显著高于肿块最大径≥5cm者(2.17±1.86)外(P<0.05),Arom和Surv在PHC中的表达情况与PHC其他主要临床病理特征之间均无明显关系;癌组织中Arom与Surv的表达评分呈正相关(r=0.316,P<0.05)。结论Arom和Surv的表达在PHC发生、发展中可能有着密切的关系。

Objective To investigate the expressions of aromatase （Arom） and survivin （Surv） in primary hepatocarcinoma（PHC）, and explore their relationships with the clinicopathology of PHC. Methods The specimens from 47 patients with PHC were fixed in 10% formalin and routinely embedded in paraffin. The specimens were continuously sliced into 4 μm-thick sections. ABC immunohistochemistry was performed to detect the expressions of Arom and Surv with polyclonal antibodies and scored them under high-power microscopy. Results The positive rates and the scores of Arom and Surv in cancer tissues were significantly higher than those of the para- tumor tissues [Arom： 40.43% vs 21.28% （P〈0.05）, 1.53± 1.69 vs0.79±1.41 （P〈0.05）; Surv： 63.83% vs 31.91% （P〈0.01）, 2.40± 1.96 vs 1.45± 1.80 （P〈0.05）3. The score of Surv in tumors with the maximal diameter 〈5 cm （4. 00 ± 2. 10） was significantly higher than that in tumors with the maximal diameter ≥ 5 cm （2.17±1.86）, P〈0.05. However, there was no relationship between the expressions of Arom and Surv in PHCs and other clinicopathologic features of the PHCs. The positive correlation was found between the score of Arom and that of Surv in PHCs （r=0. 316,P〈0.05）. Conclusion The expressions of Arom and Surv might be closely related to the carcinogenesis and development of PHC.