壳聚糖胶原支架复合胰岛素样生长因子-1在牙周组织工程中的应用

Application of Chitosan/Collagen Scaffold Containing Recombined Human IGF-1 in Periodontal Tissue Engineering

目的:观察复合胰岛素样生长因子-1(IGF-1)的壳聚糖胶原支架对体外培养的人牙周韧带细胞(PDLC)的影响,以探讨复合IGF-1的壳聚糖胶原支架在人PDLC增殖中的作用。方法:将体外培养PDLC接种于复合IGF-1的壳聚糖胶原支架上,通过MTT法测定对细胞增殖的影响,同时通过半定量PCR检测细胞mRNA合成变化。结果:PDLC在两种不同支架上的增殖及mRNA合成情况有显著性差异(P<0.5)。结论:该复合支架可以作为IGF-1的载体;复合在支架上的IGF-1对PDLC的粘附与增殖及细胞外基质的合成均有较好地促进作用。

Objective： To study IGF-1 combined chitosan/collagen scaffold and to observe the biological active of human periodontal ligament cells （PDLCs） cultured on in the scaffold. Methods： Proliferation of PDLCs was examined by MTT method when PDLCs were cultured in chitosan/collagen scaffolds with and without IGF-1 and the effect of IGF-1 combined scaffold on mRNA synthesis examined by semi-quantitive RT-PCR. Results： The proliferation and ECM synthesis of PDLCs had significant differences when cultured in different types of scaffolds. Conclusion： The chitosan/collagen scaffold could be a good controlled released system of IGF-1 and the complex scaffolds have significantly effects on proliferation of PDLCs and ECM synthesis.