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釉原蛋白基因表达质粒PcDNA3.1-AMG在小鼠肌肉组织中表达的研究

Study on Expression of Recombinant Plasmid PcDNA3.1-AMG for Human Amelogenin in Muscle Tissues of Mice
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摘要 目的研究釉原蛋白(amelogenin,AMG)重组质粒PcDNA3.1AMG能否在小鼠肌肉组织中获得正确表达。方法采用裸质粒直接注射法将溶于200μL磷酸缓冲液中的100μg质粒PcDNA3.1(对照组)或PcDNA3.1AMG(实验组)分别导入Balb/C小鼠的胫前肌内,注射后第3、7、14天分批处死小鼠,采用免疫组织化学染色法检测肌肉组织内釉原蛋白的表达。结果注射PcDNA3.1AMG的肌肉组织的肌细胞和细胞间质中均检测到釉原蛋白的表达;而注射PcDNA3.1的肌肉组织的肌细胞和细胞间质中均未检测到釉原蛋白的表达。结论PcDNA3.1AMG成功地转染了肌肉细胞,并且外源基因能够在肌细胞内转录、翻译并正确表达釉原蛋白。 Objective To investigate whether the recombinant plasmid PcDNA3.1-AMG for amelogenin could be expressed in muscle tissues of mice. Methods 100 μg naked plasmid PcDNA3.1 (control) or PcDNA3.1-AMG in 200 μ1 phosphate buffer were directly injected into the tibialis anterior muscles of two groups of Balb/C mice respectively. The mice were sacrificed after 3, 7, 14 days, and immtmohistochemical technique was applied to detect the recombinant amelogenin. Results The amelogenin was detected in the cells and interstitial spaces of the PcDNA3.1-AMG injected muscles while not in the PcENA3.1 injected muscles. Conclusion Muscle tissues could be transfected by PcDNA3.1-AMG in vivo and express recombinant amelogenin properly.
出处 《广东牙病防治》 2006年第2期105-107,共3页 Journal of Dental Prevention and Treatment
基金 广东省自然科学基金重点项目(36946) 广东省医学科学技术研究基金(A2005255)
关键词 釉原蛋白 重组质粒 转染 基因表达 Amelogenin Recombinant plasmid Transfection Expression
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参考文献10

  • 1NISHIKAWA M,HUANG L.Nonviral vectors in the new millennium:delivery barriers in gene transfer.Hum Gene Ther,2001,20(8):861-870.
  • 2章锦才,殷春一,张蕴惠,赵川江.人釉原蛋白基因的扩增及其真核表达克隆的构建[J].四川大学学报(医学版),2004,35(1):8-10. 被引量:6
  • 3徐琛蓉,杨爱玲,章锦才广东省口腔医院口腔内科,殷春一,张蕴惠,张静仪.猪发育期牙胚釉原蛋白的分离纯化及其多克隆抗体的制备[J].华西口腔医学杂志,2003,21(2):130-132. 被引量:5
  • 4FROUM S J,WEINBERG M A,ROSENBERG E,et al.A comparative study utilizing open flap debridement with and without enamel matrix derivative in the treatment of periodontal intrabony defects:a 12-month re-entry study.J Periodontology,2001,72(1):25-34.
  • 5OKUDA K,MOMOSE M,MIYAZAKI A,et al.Enamel matrix derivative in the treatment of human intrabony osseous defects.J Periodontology,2000,71(12):1821-1828.
  • 6RASPERINI G,RICCI G,SILVESTRIM,et al.Surgical technique for treatment of intrabony defects with enamel matrix derivative(Emdogain):3 case reports.Int J Periodontics Restorative Dent,1999,19(6):578-587.
  • 7ZHANG G,BUDKER V,WILLIAMS P,et al.Efficient expression of naked dna delivered intraarterially to limb muscles of nonhuman primates.Hum Gene Ther,2001,12(4):427-438.
  • 8MIAO C H,THOMPSON A R,LOEB K,et al.Long-term and therapeutic-level hepatic gene expression of human factor Ⅸ after naked plasmid transfer in vivo.Mol Ther,2001,3(6):947-957.
  • 9SAVVA M,TORCHILIN P,HUANG L.Effect of polyvinyl pyrrolidone on the thermal phase transition of 1,2 dipalmitoyl-sn-glycero-3-phospho-choline bilayer.J Colloid Interface Sci,1999,217(1):160-165.
  • 10HARTIKKA J,BOZOUKOVA V,JONES D,et al.Sodium phosphate enhances plasmid DNA expression in vivo.Gene Ther,2000,7(14):1171-1182.

二级参考文献7

  • 1[1]Brookes SJ, Robinson C, Kirkham J, et al. Biochemistry and molecular biology of amelogenin proteins of developing dental enamel. Archs Oral Biol, 1994; 40(1): 1.
  • 2[2]Gibson CW, Collier PM, Yuan ZA, et al. DNA sequences of amelogenin genes provide clues to regulation of expression. Eur J Oral Sci, 1988; 106(Suppl 1):292.
  • 3[3]David LC, John MW. Biological mediators for periodontal regeneration. Periodontology, 2000; 1999; 19:40.
  • 4[4]Wozney JM. The potential role of bone morphogenetic proteins in periodontal reconstruction. J Periodontol, 1995; 66(6):506.
  • 5[5]Lee MB. Bone morphogenetic proteins: background and implications for oral reconstruction-a review. J Clin Periodontol, 1997; 24(6):355.
  • 6[6]Gestrelius S, Andersson C, Jonhasson AC, et al. Formulation of enamel matrix derivative for surface coating. Kinetics and cell colonization. J Clin Periodontol, 1997; 24(9 Pt 2):678.
  • 7[7]Hammarstrom L. Enamel matrix, cementum development and regeneration. J Clin Periodontol, 1997; 24(9 Pt 2):658.

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