摘要
目的研究釉原蛋白(amelogenin,AMG)重组质粒PcDNA3.1AMG能否在小鼠肌肉组织中获得正确表达。方法采用裸质粒直接注射法将溶于200μL磷酸缓冲液中的100μg质粒PcDNA3.1(对照组)或PcDNA3.1AMG(实验组)分别导入Balb/C小鼠的胫前肌内,注射后第3、7、14天分批处死小鼠,采用免疫组织化学染色法检测肌肉组织内釉原蛋白的表达。结果注射PcDNA3.1AMG的肌肉组织的肌细胞和细胞间质中均检测到釉原蛋白的表达;而注射PcDNA3.1的肌肉组织的肌细胞和细胞间质中均未检测到釉原蛋白的表达。结论PcDNA3.1AMG成功地转染了肌肉细胞,并且外源基因能够在肌细胞内转录、翻译并正确表达釉原蛋白。
Objective To investigate whether the recombinant plasmid PcDNA3.1-AMG for amelogenin could be expressed in muscle tissues of mice. Methods 100 μg naked plasmid PcDNA3.1 (control) or PcDNA3.1-AMG in 200 μ1 phosphate buffer were directly injected into the tibialis anterior muscles of two groups of Balb/C mice respectively. The mice were sacrificed after 3, 7, 14 days, and immtmohistochemical technique was applied to detect the recombinant amelogenin. Results The amelogenin was detected in the cells and interstitial spaces of the PcDNA3.1-AMG injected muscles while not in the PcENA3.1 injected muscles. Conclusion Muscle tissues could be transfected by PcDNA3.1-AMG in vivo and express recombinant amelogenin properly.
出处
《广东牙病防治》
2006年第2期105-107,共3页
Journal of Dental Prevention and Treatment
基金
广东省自然科学基金重点项目(36946)
广东省医学科学技术研究基金(A2005255)
关键词
釉原蛋白
重组质粒
转染
基因表达
Amelogenin Recombinant plasmid Transfection Expression