用中效原理分析维生素K_2与顺铂对人肺癌SPC-A-1细胞株的协同作用

Synergetic cytotoxicity of vitamin K_2 and cisplatin on human lung adenocarcinoma cell line SPC-A-1

目的:研究维生素K2对人肺癌SPC-A-1细胞株的细胞毒作用及其与顺铂(Cisplatin)联合应用的相互作用。方法:MTT比色法观察维生素K2单独及与顺铂联合应用在体外对肺癌SPC-A-1细胞株的抑制作用,利用中效方程分析两药协同效应。结果:维生素K2与顺铂单用及合用时随着药物浓度增加其效应肿瘤抑制率(fa)也增加,两药合用时的中效浓度小于两药单用时的中效浓度之和,且两药无排斥性。随着维生素K2浓度的增加,其fa逐渐降低,合用指数(CI)逐渐降低,当与浓度为0．01μg／ml的顺铂联合应用,维生素K2浓度>18．57μg/ml时,两药即可产生协同作用。结论:维生素K2与顺铂联合应用优于单药对SPC-A-1细胞株的抑制作用。

Objective： To investigate the suppressive effects of vitamin K2 on human adenocarcinoma cell line SPC-A-1 and the interaction between vitamin K2 and cisplatin. Methods： The cytotoxic indexes of vitamin K2 alone or combined with cisplatin on SPC-A-1 cells were detected by MTT method. Results： SPC-A-1 was sensitive to vitamin K2 with a positive correlation between cytotoxic index and vitamin K2 concentration. There were significant synergetic effects in cytotoxicity of vitamin. K2 combined with cisplatin on SPC-A-1 cells. Conclusion： Vitamin K2 is a promising agent in the treatment of human lung cancer.