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汉坦病毒G1和G2糖蛋白的基因克隆及糖蛋白杆状病毒表达载体的构建 被引量：2

Gene cloning of glycoprotein of Hantavirus and construction of G1 and G2 glycoprotein baculovirus expression vectors
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摘要 目的:克隆汉坦病毒G1和G2糖蛋白基因,构建基于杆状病毒表面展示系统(BSDS)的G1和G2糖蛋白杆状病毒表达质粒。方法:用PCR方法扩增汉坦病毒西安分离毒洙84FLi株G1和G2糖蛋白基因的编码区,将其克隆入T载体,构建糖蛋白基因的T-A克隆。然后将糖蛋白编码区从T-A克隆中切下,插入杆状病毒表达转移载体pBAcsurf-1的gp64信号肽和gp64成熟蛋白之间。结果:获得四个分别含有汉坦病毒G1和G2糖蛋白编码区的T-A克隆,并构建成功G1和G2糖蛋白的杆状病毒表达质粒。结论:构建成功基于BSDS的G1和G2糖蛋白杆状病毒表达质粒,为进一步用BSDS表达汉坦病毒糖蛋白奠定了基础。 Objective：To clone the genes of G1 and G2 glycoprotein of Hantavirus and construct recombinant G1 and G2 glycoprotein expression vectors based on baculovirus surface display system. Methods ： The open reading frame of G1 and G2 glycoprotein of 84 FLi, a Hantavirus isolated in Xi＇ an, was amplified and cloned into pGEM-T Easy Vector to produce T-A clones. The gene of glycoprotein was digested from the T-A clones and inserted into transfer vector pBACsurf-1 between the upstream gp64 signal sequence and downstream gp64 mature domain. Results：Four T-A clones containing the open reading frame of G1 and G2 glycoprotein of 84 FLi were constructed and the G1 and G2 glycoprotein baculovirus expression vectors were also constructed successfully. Conclusion：Recombinant G1 and G2 glyeoprotein expression vectors based on baeulovirus surface display system were constructed and which make it possible to further express the glyeoprotein.
作者 李新红 张岩 张野 王平忠 李光玉 黄长形 白雪帆
机构地区 第四军医大学唐都医院解放军感染病诊疗中心
出处 《医学研究生学报》 CAS 2006年第5期405-408,413,共5页 Journal of Medical Postgraduates
基金 国家自然科学基金资助项目(批准号:30200010)
关键词 汉坦病毒 糖蛋白 基因克隆 杆状病毒表面展示系统 Hantavirus Glyeoprotein Gene cloning Baeulovirus surface display system
分类号 Q785 [生物学—分子生物学]
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参考文献21

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医学研究生学报
2006年 第5期

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