期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

糖尿病大鼠肾病变过程中核因子-κB及诱导型一氧化氮合酶的表达 被引量：6

Diabetes-associated sustained activation of nuclear factor-κB and inducible nitric oxide synthase in the kidneys of diabetic rats
下载PDF
导出
摘要 目的:探讨糖尿病肾病变与核因子-κB活化及诱导型一氧化氮合酶(iNOS)表达的关系。方法:雄性SD 大鼠60只,随机分成对照1、3、6个月组和糖尿病1、3、6个月组,每组10只。分别于1、3和6个月末处死大鼠,留取肾标本,观测肾脏病理改变并用免疫组化法分析NF-κB及iNOS表达。结果:肾病理结果显示,与对照组相比, 糖尿病1、3、6个月组肾病理改变逐步加重;免疫组化分析结果显示NF-κB和iNOS表达在糖尿病各组均明显增强 (P<0．05)。结论:NF-κB及iNOS伴随着肾病理学改变而持续活化,可能在糖尿病肾病(DN)发生中有重要作用, 可作为反映进行性肾损害的参考指标。 Objective： Activation of the transcription factor nuclear faetor-kappaB （ NF-κB ） has been suggested to participate in chronic disorders, such as diabetes and its complications. In contrast to the short activation of NF-κB , we observed a long-lasting activation of NF-κB and iNOS in the kidneys of diabetic rats. Methods： Sprague-Dawley rats were randomly divided into control group and diabetic group. The pathological changes in the kidneys of diabetic rats were observed at 1, 3 and 6 months respectively. The activity of NF-κB and iNOS was detected by immunohistoehemistry. Results： The change of histopathology was severer at 6 months than at 3 months and 1 month in the kidneys of diabetic rats. A comparable increase in NF-κB p65 antigen was observed in the kidney cells especialy in vascular endothelial, cells of diabetic rats, and the expression of iNOS in renal tissues of diabetic rats was higher than that of normal control. Conclusion ： NF-κB and iNOS may play an important role in the pathogeneis of diabetic rats, and the expression of them may be considered as a predictor of progressive renal injury in diabetic rats.
作者 王扬天 赵明 狄红杰
机构地区 南京大学医学院临床学院南京军区南京总医院内分泌科
出处 《医学研究生学报》 CAS 2006年第5期416-419,共4页 Journal of Medical Postgraduates
基金 南京军区南京总医院科研基金资助项目(批准号:2005036)
关键词 核因子-ΚB 诱导型一氧化氮合酶 糖尿病肾病 Nuclear factor-κB Inducible nitric oxide synthase Diabetic nephropathia
分类号 R587.1 [医药卫生—内分泌] R692 [医药卫生—泌尿科学]
  • 相关文献

参考文献8

  • 1Hofmann MA,Schiekofer S,Isermann B,et al.Peripheral blood mononuclear cells isolated from patients with diabetic nephropathy show increased activation of the oxidative-stress sensitive transcription factor NF-κB[J].Diabetologia,1999,42 (2):222-232.
  • 2卢海,王坚.噻唑烷二酮类药物对糖尿病肾病的直接作用及其机制[J].医学研究生学报,2004,17(8):756-759. 被引量：7
  • 3Lentsch AB,Kato A,Yoshidome H,et al.Inflammatory mechanisms and therapeutic strategies for warm hepatic ischemia-reperfusion injury[J].Hepatology,2000,32 (2):169-173.
  • 4Lemay S,Rabb H,Postler G,et al.Prominent and sustained upregulation of gp130-signaling cytokines and of chemokine MIP-2 in murine renal ischemia/reperfusion injury[J].Transplantation,2000,69 (5):959-963.
  • 5Ho E,Bray TM.Antioxidants,NF-κB activation and diabetogenesis[J].Proc Soc Exp Biol Med,1999,222(3):205-213.
  • 6Papaccio G,Graziano A,Aquino R,et al.A biphasic role of nuclear transcription factor (NF) -kappaB in the islet beta-cell apoptosis induced by interleukin (IL)-1beta[J].J Cell Physiol,2005,204(1):124-130.
  • 7Poljakovic M,Nygren JM,Persson K.Signalling pathways regulating inducible nitric oxide synthase expression in human kidney epithelial cells[J].Eur J Pharmacol,2003,469 (1-3):21-28.
  • 8Nishigaki R,Guo F,Onda M,et al.Ultrastructural changes and immunohistochemical localization of nitric oxide synthase,advanced glycation end products and NF-kappaB in aorta of streptozotocin treated Mogolian gerbils[J].Nippon Ika Daigaku Zasshi,1999,66(3):166-175.

二级参考文献22

  • 1[1]Fujii M, Takemura R, Yamaguchi M et al. Troglitazone (CS-045) ameliorates albuminuria in streptozotocin-induced diabetic rats[J]. Metabolism, 1997,46(9):981-983.
  • 2[2]Isshiki K, Haneda M, Koya D et al. Thiazolidinedione compounds ameliorate glomerular dysfunction independent of their insulin-sensitizing action in diabetic rats[J]. Diabetes, 2000, 49(6):1022-1032.
  • 3[3]Yamashita H, Nagai Y, Takamura T et al. Thiazolidinedione derivatives ameliorate albuminuria in streptozotocin-induced diabetic spontaneous hypertensive rat[J]. Metabolism, 2002, 51(4):403-408.
  • 4[4]Robert RE, John JH, McCarthy KJ. Troglitazone suppresses the secretion of type I collagen by mesangial cells in vitro[J]. Kidney Int, 2002, 61(4): 1365-1376.
  • 5[5]Nakamura T, Ushiyama C, Suzuki S et al. Effect of troglitazone on urinary albumin excretion and serum type Ⅳ collagen concentrations in Type 2 diabetic patients with microalbuminuria or macroalbuminuria[J]. Diabet Med, 2001,18(4):308-313.
  • 6[6]Haneda M, Koya D, Kikkawa R. Cellular mechanisms in the development and progression of diabetic nephropathy: activation of the DAG-PKC-ERK pathway[J]. Am J Kidney Dis, 2001, 38(4 Suppl 1):S178-181.
  • 7[7]Isono M, Iglesias-de la Cruz MC, Chen S et al. Extracellular signal-regulated kinase mediates stimulation of TGF-β1 and matrix by high glucose in mesangial cells[J]. J Am Soc Nephrol, 2000,11(12):2222-2230.
  • 8[8]Haneda M, Araki S, Togawa M et al. Mitogen-activated protein kinase cascade is activated in glomeruli of diabetic rats and glomerular mesangial cells cultured under high glucose conditions[J]. Diabetes, 1997,46(5): 847-853.
  • 9[9]Sakamoto K, Kikkawa R, Haneda M et al. Prevention of glomerular hyperfiltration in rats with streptozotocin-induced diabetes by an atrial natriuretic peptide receptor antagonist[J]. Diabetologia,1995,38(5):536-542.
  • 10[10]Suzuki E, Hirata Y, Kohmoto O et al. Cellular mechanisms for synthesis and secretion of atrial natriuretic peptide and brain natriuretic peptide in cultured rat atrial cells[J]. Circ Res, 1992,71(5):1039-1048.

共引文献6

同被引文献100

  • 1王晓,李果,李纪平,刘赟.泛素在高糖诱导的血管内皮细胞凋亡中的表达[J].上海第二医科大学学报,2005,25(1):36-38. 被引量：7
  • 2狄红杰,赵明,王扬天.糖尿病大鼠视网膜病变核因子-κB表达水平的研究[J].医学研究生学报,2006,19(4):341-343. 被引量：8
  • 3王金泉,刘志红.终末期糖尿病肾病肾替代治疗[J].医学研究生学报,2006,19(7):664-668. 被引量：20
  • 4金建生,苏波峰,叶洪.糖尿病大鼠肾皮质细胞过度凋亡[J].福建医科大学学报,2006,40(5):447-451. 被引量：1
  • 5Browlee M. The pathobiology of diabetic complications, a unifying mechanism [ J]. Diabetes, 2005, 54(6) :1615-1625.
  • 6Chander PN, Gealekman O, Brodsky SV, et al. Nephropathy in zucker diabetic fat rat is associated with oxidative and nitrosative stress: prevention by chronic therapy with a peroxynitrite scavenger ebselen[J]. J Am Soc Nephrol, 2004, 15(9) : 2391-2403.
  • 7Catherwood MA, Powell LA, Anderson P, et al. Glucose-induced oxidative stress in mesangial cells[ J ]. Kidney Int, 2002, 61 (2) :599-608.
  • 8Kakimoto M, Inoguchi T, Sonta T, et al. Accumulation of 8-Hydroxy- 2-deoxyguanosine and mitochondrial DNA deletion in kidney of diabetic rats [ J ]. Diabetes, 2002, 51 (5) : 1588-1595.
  • 9Craven PA, Melham MF, Phillips SL, et al. Overexpression of Cu^2+/Zn^2+ superoxide dismutase protects against early diabetic glomerular injury in transgenic mice[J]. Diabetes, 2001, 50 (9) :2114-2125.
  • 10Gorin Y, Block K, Hemandez J, et al. Nox4 NAD(P)H oxidase mediates hypertrophy and fibroneetin expression in the diabetic kidney[ J] . J Biol Chem, 2005, 280(47) : 39616 -39626.

引证文献6

二级引证文献96

医学研究生学报
2006年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部