生存素和细胞周期蛋白在非小细胞肺癌组织中的表达

Expression of survivin and cyclin D1 protein in human non-small cell lung carcinoma

目的:研究生存素(survivin)和细胞周期蛋白(cyclin)D1在非小细胞肺癌(NSCLC)组织中的表达及其生物学意义,并探讨其相关性。方法:采用免疫组化S-P法检测45例NSCLC组织中surivin和cyclin D1的表达。结果:survivin蛋白在正常肺组织中不表达,45例NSCLC组织中27例阳性表达,占60％,其表达与分化程度、临床分期、淋巴结转移密切相关,但与肿瘤组织学类型、性别、年龄无关。NSCLC组织中survivin和cyclin D1蛋白表达两者比较有显著性差异(P<0．05)。结论:survivin基因在肺癌组织中表达上调,提示该基因与肺癌的发生、发展密切相关;survivin基因可能与cyclin D1共同在NSCLC细胞有丝分裂中起促进作用。survivin和cyclin D1可作为靶点进行肺癌靶向治疗的研究。

Objective： To research the expression of survivin and cyclin D1 in human lung cancer, and to illustrate their relationship in NSCLC. Methods： Forty-five NSCLC non-small cell paraffin embeded samples were collected. Survivin and cyclin D1 were tested by immunohistochemical S-P method. Results： No survivin expression was present in normal lung tissues. The positive rate of survivin in NSCLC was 60% （27/45）. By statistic analysis, the significant differences were found in different pathological grading and clinical phased lymph node involvement. The patient＇ s gender, age and histological classification were not related with the expression of survivin. Conelusion：Survivin may play an importment role in the process of carcinogenesis and development of NCLC. Survivin and cyclin D1 might play synergetic roles in lung cancer cell＇ s karyokinesismitosis and they can be identified as potential therapeutic target in NSCLC.