男性不育患者精液质量与精子顶体酶活性关系分析

Analysis of Relationship between Semen Quality and Sperm Acrosin Activity

目的：分析男性不育患者精液参数与精子顶体酶活性变化，探讨精液质量与顶体酶活性的关系。方法：对214例男性不育患者的精液作精子顶体酶活性、弹性蛋白酶、果糖、α葡糖苷酶、锌、酸性磷酸酶、精子尾部低渗肿胀试验检测，同时精子质量检测仪作精液分析。以检出精子顶体酶活性正常的（48．2-218．7μIU／10^6精子）111例作为对照组，与顶体酶活性异常（〈48．2μIU／10^6精子）的103例精液参数作对比分析。结果：两组精液参数的精子密度、精子活动率、a＋b级精子百分率、精子尾部低渗肿胀试验差异均有非常显著性（P〈0．001）。弹性蛋白酶差异有显著性（P〈0．05）。果糖、α葡糖苷酶差异亦有显著性（P〈0．05）。精液量、锌、酸性磷酸酶差异无显著性（P〉0．05）。结论：精子顶体酶活性与精液质量关系密切，精子顶体酶活性是反映精液质量的一项可靠指标。

Objective： To analyze the activity of human sperm acrosin and semen parameters in male infertile patients and discuss the effect of sperm acrosin activity on semen quality. Methods ： Activity of human sperm acrosin, PMN-elastase, fructose, α-glucosidase, zinc, acid phosphatase levels and HOST of 214 male infertile patients were detected. Semen analysis was performed using WLJY9000 WeiLi colorful semen quality analyzer. There were 111 cases of normal activity of human sperm acrosin （48.2 - 218.7 μIU/10^6 sperm）, 103 cases abnormal（ 〈48.2 μIU/10^6 sperm）. Using the group of normal activity of sperm acrosin to be the control, semen parameters was analyzed and compared with those of the group of abnormal activity of sperm acrosin. Results： There were significant difference （P 〈0.001 ） between the 2 groups（ normal and abnormal） in the areas of sperm density, motile sperm rate, percentage of grade （ a ＋ b） sperm and HOST. There were also significant difference in PMN-elastase, fructose and α-glucosidase（P 〈 0.05 ）. There was no difference among sperm volume, zinc and acid phosphatase （ P 〉 0.05 ）. Conclusion ： There was a strong correlation between the activity of human sperm sperm acrosin and semen quality. Activity of sperm acrosin is a reliable index of semen quality.