HLA-G蛋白在人类着床前胚胎的表达

Expression of HLA-G Protein in Human Preimplantation Embryos

目的:探讨HLA-G在人类胚胎早期发育和免疫耐受中的作用和意义。方法:以体外受精-胚胎移植技术助孕过程中剩余的、自愿捐赠的人类早期胚胎作为研究对象,采用免疫荧光染色技术,检测人类着床前胚胎HLA-G蛋白的表达。结果:采用MEM-G/9单抗进行了HLA-G免疫荧光染色,20个2-8-细胞期的人类早期胚胎中15个胚胎HLA-G蛋白检测阳性;3个桑椹胚和5个囊胚均为阳性。结论:着床前人类早期胚胎表达HLA-G蛋白。

Objective： To investigate the function and significance of human leukocyte antigen-G（HLA-G） protein in human preimplantation embryos and immunological tolerance. Methods： Human preimplantation embryos,which were residual and contributed, were obtained during assisted conception process by IVF-ET. lmmunofluorescence staining was used to detect HLA-G protein in varies stages of human preimplantation embryos.Results： Twenty 2-cell to 8-cell human embryos were stained with MEM-G/9, a monoclonal antibody specific for HLA-G. HLA-G labeling was found in 15/20 2-cell to 8-cell human embryos. HLA-G positive labeling was observed in all 3 morulas and 5 blastocysts. Conclusions： Human preimplantation embryos express HLA-G protein.