sgp190与白血病抑制因子对滋养层细胞金属蛋白酶-9及抑制因子-1表达的影响

Study on the Effect of sgp190 and LIF on MMP-9/TIMP-1 Production in Human Trophoblasts

目的:探讨sgp190与白血病抑制因子(LIF)在妊娠维持中的作用。方法:体外培养滋养层细胞,分别施加LIF和/或sgp190处理后,用RT-PCR法检测金属蛋白酶-9(MMP-9)与金属蛋白酶组织抑制因子(TIMP-1)mRNA表达情况,免疫印迹法检测细胞MMP-9与TIMP-1蛋白表达水平,ELISA法测定培养上清中MMP-9与TIMP-1的浓度。结果:LIF抑制体外培养滋养层细胞中MMP-9与TIMP-1中mRNA和蛋白表达(P<0.05),而sgp190可抑制LIF的这种作用。sgp190促进MMP-9mRNA与蛋白的表达(P<0.05),但对TIMP-1mRNA与蛋白表达无影响。ELISA分析结果基本与RT-PCR和免疫印迹分析结果相同。结论:LIF和sgp190可能通过调节MMPs和TIMPs表达来影响滋养层细胞的侵袭,而sgp190在LIF功能活性调节中扮演着一定角色。

Objeetive： To investigate whether sgp190 and/or LIF play an important role during pregnancy.Methods： After trophoblasts were cultured for 24 h in vitro, the cells were treated with medium containing 10 ng/ml LIF, 30 ng/ml sgp190, and combination of LIF and sgp190, respectively. Trophoblasts were harvested after being treated for 48 h, and mRNA and protein expression levels of MMP-9 and TIMP-1 in trophoblasts were detected by RT-PCR and Western blotting, respectively. The culture media were collected for quantitation of MMP-9 and TIMP-1 by ELISA. Results： LIF could inhibit MMP-9 and TIMP-1 mRNA and protein expression in trophoblasts,and sgp190 could block the effect of LIF. Sgp190 boosted up the abundance of MMP-9 mRNA and protein, but had no effect on TIMP-1. The results of ELISA was similar to that of RT-PCR and Western blotting. Conclusion： LIF and sgp190 could influence the invasion ability of trophoblasts through regulating MMPs and TIMPs expression,and sgp190 may be involved in the biological regulation of LIF.