期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

Toll样受体3参与双链RNA对小鼠妊娠结局的影响 被引量：1

下载PDF
导出
摘要 目的:探讨Toll样受体3(TLR3)与双链RNA(dsRNA)相互作用对BALB/c和NOD/SCID小鼠妊娠结局的影响。方法:比较经过和未经预先TLR3阻断条件下同基因妊娠的BALB/c和NOD/SCID小鼠在dsRNA刺激后的胚胎吸收率(RR),并采用流式细胞术检测CD45+TLR3+细胞和CD45+CD80+细胞在CD45+细胞群中、以及CD8α+CD80+细胞在CD8α+细胞群中的百分率。结果:在dsRNA刺激下BALB/c小鼠RR显著升高,而对NOD/SCID小鼠无显著影响。BALB/c小鼠胎盘CD45+细胞群中TLR3表达率升高,并伴有CD45+CD80+细胞和CD8α+CD80+细胞百分率的升高。另可检测到CD45+细胞内IL-2表达率增高,而IL-10表达率下降;而在NOD/SCID模型中未观察到这种趋势。阻断TLR3能消除dsRNA对BALB/c小鼠胚胎排斥的诱导作用,并同时降低CD45+细胞群中CD80的表达率。结论:dsRNA与TLR3的相互作用可能参与CD45+CD80+细胞和CD8α+CD80+细胞的动员,随后母-胎界面IL-2表达增强,而IL-10表达减弱,最终导致胚胎被排斥。dsRNA引起的胚胎排斥过程可能需要具有正常功能的T细胞和NK细胞参与。
作者 林羿 张家玮 狄静芳 杨志 陈艺菁 曾耀英
机构地区 暨南大学组织移植与免疫教育部重点实验室
出处 《生殖与避孕》 CAS CSCD 北大核心 2006年第5期315-319,共5页 Reproduction and Contraception
基金 中国博士后科学基金(2002032244) 广东省自然科学基金(4300213) 暨南大学引进优秀人才基金(51204067) "211工程"母-胎界面免疫学研究基金项目.
关键词 动物模型 免疫缺陷 妊娠耐受 TOLL样受体
分类号 R392 [医药卫生—免疫学] R714 [医药卫生—妇产科学]
  • 相关文献

参考文献2

二级参考文献15

  • 1Bosma GC, Custer RP, Bosma MJ. A severe combined immunodeficiency mutation in the mouse. Nature, 1983, 301: 527-530.
  • 2Dorshkind K, Pollack SB, Bosma MJ, et al. Natural killer(N'K) cells are present in mice with severe combined immunodeficiency(seid). J Immunol, 1985, 134: 3798-3801.
  • 3Kataoka S, Satoh J, Fujiya H, et al. Immunologic aspects of the nonobese diabetic(NOD) mouse: Abnormalities of cellular immunity. Diabetes, 1983, 32: 247-253.
  • 4Christianson SW, Shultz LD, Leiter EH. Adoptive transfer of diabetes into immunodeficient NOD-scid/scid mice: relative contributions of CD4^+and CD8^+ T ly-mphocytes frmn diabetic versus prediabetic NOD. NON-Thy-1^a donors. Diabetes, 1993, 42: 44-55.
  • 5Shultz LD, Schweitzer PA, Christianson SW, et al. Multiple defects in innate and adaptive immunologic function in NOD/LtSz-scid mice. J Immunol, 1995, 154: 180-191.
  • 6Christianson SW, Greiner DL, Hesselton R, et al. Enhanced human CD4^+ T cell engraftment in β2-microgtobulin-deficient NOD-scid mice. J Immunol, 1997, 158: 3578-3586.
  • 7Tempfer CB, Moreno RM, Gregg AR. Genetic control of fertility and embryonic waste in the mouse: a role for angiotensinogen. Biol Reprod,2000, 62: 457-462.
  • 8Shimada S, Iwabuchi K, Watano K, et al. Expression of allograft inflammatory factor-1 in mouse uterus and poly (I:C)-indueed fetal resorption.Am J Reprod Immunol, 2003, 50: 104-112.
  • 9Lee I-F, Qin H, Trudean J, et al. Regulation of autoimmune diabetes by complete Freund's adjuvant is mediated by NK cells. J Immunol, 2004,172: 937-942.
  • 10Clark DA, Chaouat G, Arck PC, et al. Cytokine-dependent abortion in CBA x DBA/2 mice is mediated by the procoagulant fgl2 prothrombinase.J Imnamol, 1998, 160: 545-549.

共引文献9

同被引文献9

  • 1Hashimoto C,Hudson KL,Anderson K et al.The Toll gene of Drosophila,required for dorsal ventral embryonic polarity,appears to encode a transmembrane protein.Cell,1988,52(2):269.
  • 2Medzhitov R,Preston HP,Janeway CA.A human homologue of the Drosophila Toll protein signals activation of adaptive immunity.Nature,1997,388 (6640):394.
  • 3Patricia PA,Amiel E,Schaefer TM et al.Differential Expression of Toll-Like Receptors 2 and 4 in Tissues of the Human Female Reproductive Tract.Infection and Immunity,2004,72(10):5799.
  • 4Hirata T,Osuga Y,Hirata Y et al.Evidence for the Presence of-Toll Like Receptor 4 System in the Human Endometrium.The Journal of Clinical Endocrinology & Metabolism,2005,90(1):548.
  • 5Kim YM,Romero R,Chaiworapongsa T et al.Toll-like receptor-2 and-4 in the chorioamniotic membranes in spontaneous labor at term and in preterm parturition that are associated with chorioamnionitis.Am J Obstet Gynecol,2004,191(4):1346.
  • 6Elovitz MA,Wang Z,Chien EK et al.A New Model for Inflammation-Induced Preterm Birth:The Role of Platelet-Activating Factor and Toll-Like Receptor-4.Am J Pathol,2003,163(5):2103.
  • 7Mitsunari M,Yoshida S,Shoji T et al.Macrophage activating lipopeptide-2 induces cyclooxygenase-2 and prostaglandin E2 via toll like receptor 2 in human placental trophoblast cells.Journal of Reproductive Immunology,2006,72(1-2):46.
  • 8Jorgcnson RL,Young SL,Lesmeister MJ et a1.Human endometrial epithelial cells cyclically express Toll-like receptor 3 (TLR3) and exhibit TLR3-dependent responses to dsRNA.Ham Immunol,2005,66(5):469.
  • 9Kelly MG,Alvero AB,Silasi DA et al.TLR-4 signaling promotes tumor growth and Paclitaxel chemoresistance in ovarian cancer.Cancer research,2006,66 (7):3859.

引证文献1

二级引证文献1

生殖与避孕
2006年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部