一种孕宝营养液致畸和遗传毒性的检测

Toxicology of a Health Food for Pregnant Woman

背景与目的:研究某孕宝营养液的致畸性和遗传毒性,为其应用提供安全性毒理学评价依据。材料与方法:设0.83、1.67、3.33g/kg3个剂量组,进行传统致畸研究;设8、40、200、1000和5000μg/皿5个剂量组,进行Ames试验;设2.5、5.0和10.0g/kg3个剂量组,观察其对小鼠骨髓细胞微核和小鼠精子畸形的影响。结果:3个剂量组各项指标均未见有明显的母体毒性和胚胎毒性、致畸性。各剂量组在体内体外致突变性研究中未见有遗传毒性。结论:在本实验条件下,某孕宝营养液无致畸性和遗传毒性。

BACKGROUND ＆ AIM： To study the teratogenicity and mutagenicity of a particular health food for pregnant woman, in order to assess its safety. MATERIAL AND METHODS： Teratogenicity study was set up using doses of 0.83, 1.67 and 3.33 /kg in rats, and Salmonella typhimurium/mammals microsomal enzyme test, at the doses of 8, 40,200, 1 000 and 5 000 μg/plate, bone marrow cell mironucleus test and sperm abormality test in mice, at the doses of 2.5,5.0 and 10.0 g/kg. RESULTS： There was no obvious maternal toxicity , embryotoxicity and teratogenicity,and also there was no inherited toxicity in vitro and in vivo testing system. CONCLUSION： Our study showed that this particular health food for pregnant woman had no teratogenicity nor mutagenicity.