猪传染性胸膜肺炎放线杆菌参考株抗血清的制备及野毒株血清型鉴定

Preparation of Antisera to the Reference Strains of Actinobacillus pleuropneumoniae and Serotype Identification of its Field Strains

将猪传染性胸膜肺炎放线杆菌全部12个血清型的国际参考菌株接种于培养基大量培养。回收菌体、甲醛灭活后，加入蜂胶佐剂制成疫苗。分别免疫健康的试验用白兔。获得针对该菌单一血清型的抗血清。虽然不同血清型间有一定的交叉反应，但对同源菌株的菌体抗原的凝集效价最高。分别可达6～8个log2。用该套血清对2003年从山东省各地分离到的猪传染性胸膜肺炎放线杆菌野毒株11株进行了血清型鉴定。分别为3型（2株）、4型（1株）、5型（4株）和7型（4株）。

Rabbits were inoculated with formaldehyde-inactivated bacteria suspensions of Actinobacillus pleuropneumoniae （APP）strains of 12 serotypes with bee glue as an adjuvant, to prepare a set of monospecific antisera against each serotypes of APP. Although there were some cross-reactions between the reference strains of different serotypes,any monospecific antiserum gave the highest titers to its homologous APP reference strain bacteria suspension in agglutination test,they reached titers of 6-8 log2 respectively. By use of the set of antisera ,the field strains isolated from different areas in Shandong privince in 2003 were identified for their serotypes,they belong to serotypes 3（2 isolates）,4 （1 isolates）,5（4 isolates）and 7 （4 isolates）, respectively.