2种猪源链球菌菌体细胞表面疏水性及体外结合纤连蛋白的检测

Hydrophobic Characterization and adhesion of Streptococcus equi subsp. zooepidemicus ATCC35246 and Streptococcus suis type 2 Jiangsu isolate 9801 to fibronection

采用盐析法测定了马链球菌兽疫亚种ATCC35246株(简称35246)和猪链球菌2型9801株(简称HA9801)的表面疏水性。35246在浓度为1.0mol/L的硫酸铵中2min内析出,HA9801在0.6mol/L的硫酸铵中析出。用不同浓度的人纤连蛋白(Fn)包被ELISA板,采用ELISA方法检测了对数生长后期的35246和HA9801体外结合固定Fn的情况。35246的D415随细菌浓度的升高而增大,而9801的D415与对照相比,经t检验差异不显著(P>0.05)。采用间接免疫荧光试验,对二者体外结合游离的人Fn的情况进行了检测,结果在2种细菌周围均见绿色荧光。结果表明,二菌均为表面疏水菌;35246株既可结合固定的Fn,又可结合游离的Fn;而HA9801株仅与游离的Fn结合。

Cell surface hydrophobicity of Streptococcus equi subsp . zooepidemicus ATCC35246 and Streptococcus suis type 2 jiangsu isolate HA9801 was determined by ＂salting out＂. The interaction of soluble and immobilized fibronectin with the two species was investigated by indirect immunofluorescent assay and enzyme-linked immunosorbent assay, respectively. The resuits showed that ATCC35246 and HA9801 was high cell surface hydrophobic and that the former could bind the two forms of fibronectin, but the latter bind the soluble one only.