摘要
通过应用抑制素真核表达质粒pcINH和抑制素融合表达质粒pCIS分别经肌肉注射免疫大鼠的2个系列试验,检测了免疫鼠抑制素抗体水平及抗体阳性鼠的比例。结果,抑制素质粒pcINH经脂质体介导免疫大鼠,50%(13/26)的个体产生了抑制素阳性抗体,40μg组产生的抗体水平最高;抑制素融合表达质粒pCIS免疫经盐酸普鲁卡因处理的大鼠,2次免疫获得了38.9%(23/54)的抗体阳性率,3次免疫获得55.6%(20/36)的抗体阳性率,100μg组产生的抗体水平最高;加强免疫可提高抗体的水平,但免疫剂量的增加并不一定能增加抗体阳性鼠的比例。
Two serial studies were conducted to examine the level of antibody against inhibin and the ratio of rats with positive antibody. The results showed that immunized with pcINH coated in liposome ,50% （13/26） of rats were detected in positive antibody against inhibin,and rats immunized with 40 μg produced the highest level of antibody against inhibin; immunized with pCIS after treatment of bupivacaine hydrochloride, 38.9% （23/54） of rats were detected in positive antibody against inhibin after twice immunizations, 55.6 % （20/36） of rats were detected in positive antibody against inhibin after three times immunizations,and rats immunized with 100 μg produced the highest level of antibody against inhibin. Booster immunization could raise the value of antibody against inhibin. The increase of immunization dosage wasn＇t sure to raise the ratio of rats with positive antibody.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2006年第3期292-295,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30270959)
