MTA直接盖髓的动物实验研究

An in vitro experimental study of direct pulp capping with mineral trioxide aggregate

目的比较MTA(mineral trioxide aggregate)及氢氧化钙犬牙直接盖髓后牙髓的反应。方法将三只健康雄性杂种犬共72颗牙齿机械开髓,然后用MTA和氢氧化钙分别盖髓,窝洞用玻璃离子水门汀充填。在5,7,9个月后,对实验牙齿进行组织切片制备,借助计算机图文处理系统对所形成的修复性牙本质宽度进行测量,对成牙本质细胞、牙髓细胞及毛细血管进行计数、分析。结果5个月后,MTA及氢氧化钙盖髓效果相似。在7,9个月后,MTA组牙髓较氢氧化钙组可见更明显的修复性牙本质形成,更多的成牙本质细胞和牙髓细胞。结论MTA具有临床活髓保存治疗应用价值。

Objective To compare the dental pulp responses of mineral trioxide aggregate （MTA） with calcium hydroxide when used as pulp - capping materials in dog teeth. Methods Seventy - two teeth from three healthy adult beagle dogs were subjected to mechanical pulp exposure. The exposed pulps were capped with MTA or calcium hydroxide. All the cavities were filled with glass ionomer cement （GIC）. After periods of 5 months, 7 months and 9 months, the specimens were processed . The quantitative information about the numbers of odontoblast cells, pulp cells, and capiUaries was collected. The width of reparative dentin was measured using computer - based image analysis system. Resells After 5 months, the dental pulp responses to MTA and calcium hydroxide were similar. After 7 months and 9 months, wider reparative dentin, more odontoblast cells, and pulp ceils in MTA group were seen compared to the calcium hydroxide group. Conclusion MTA is a promising material for vital pulp therapy.