镉对小鼠腹腔巨噬细胞吞噬功能影响与PGE_2关系的实验研究

Study on Relationships between Effects of Cadmium on Phagocytic Function of Mouse Peritoncal Macrophage and PGE_2

镉可影响小鼠腹腔巨噬细胞（Ｍ）对鸡红细胞的吞噬功能．为了探讨前列腺素Ｅ２（ＰＧＥ２）与这种吞噬功能的关系，经镉染毒后测定小鼠腹腔液ＰＧＥ２的水平．结果表明：投毒后２周，饮低镉组与对照组相比吞噬率、吞噬指数差异无显著性（Ｐ＞０．０５）；饮高镉组与对照组相比吞噬率、吞噬指数显著降低（Ｐ＜０．０１）；消炎痛加高镉组与单纯高镉组相比吞噬率显著增高（Ｐ＜０．０５）；加消炎痛各组ＰＧＥ２水平均低于非消炎痛组（Ｐ＜０．０１），ＰＧＥ２水平与巨噬细胞吞噬率呈负相关（Ｒ＝０．６６６３，Ｐ＜０．０１）．上述实验结果表明，高剂量镉对小鼠巨噬细胞吞噬功能具有显著抑制作用，ＰＧＥ２在镉对Ｍ吞噬功能毒作用中起重要作用．

We divided the male kunming mouse into 5 groups randly:① control ② Drinking low dose cadmium(1×10-5 mol/L)water ③ Drinking high dose cadmium (1×10-3 mol/L) ④Drinking indometbacin (1×10-4 mol/L) ⑤ Drinking indomenthacin(1×10-4 mol/L)+ cadmium (1×10-3 mol/L) water.They are exposed for tow weeks.After peritoneal injection with 1% chicken red blood cells for 1h,peritoneal fluid is taken out for smearing.The smears wter stained and counted.Some of the peritoneal fluid was used to measure PGE2.The results show that the phagocytic rate and phagoindex of mouse peritoneal maeropbage campairied with that of control didn't changed significantly in low dose camium group(P<0.05).While there was a increasing thendency.And that of high cadmium dose group decreased significantly as compaired with that of control(P<0.01). Indomethacin+ high dose cadmium group compaired with the group exposed to high dose cadmium.the phagocytic rate is higher in significance.The PGE2 of the groups with indomethacin decreased significantly(P<0.01).The results indicat that PGE2 plays an important role in the mechanism of immunotoxicity of cadmium.