Survivin蛋白在喉鳞癌组织中的表达及其与bcl-2表达的关系

Expression and biological significance of survivin and Bcl-2 in laryngeal squamous cell carcinoma

目的探讨凋亡抑制基因survivin蛋白在喉鳞癌中的表达,及其与Bcl-2表达的相关性。方法对47例喉鳞癌组织和癌旁组织及正常喉组织的石蜡切片,采用免疫组织化学技术SP法检测survivin蛋白及Bcl-2蛋白的表达。结果survivin在正常喉组织中不表达,survivin在喉鳞癌、癌旁组织中的阳性表达率分别为59.6%(28/47)和25.5%(12/47)。高分化喉鳞癌中survivin的阳性表达率为33.3%,中、低分化为68.6%,两者比较差异有显著性;临床分期III、IV期喉癌中survivin阳性率明显高于临床分期I、II期;有颈淋巴结转移病例的survivin阳性表达率(61.9%)明显高于无颈淋巴结转移病例(30.0%,P<0.05);survivin与临床分期、病理分级、淋巴结转移呈正相关。Bcl-2表达阳性、阴性者中,survivin蛋白表达阳性率分别为78.3%(18/23)、41.7%(10/24),两者比较,差异有显著性。结论Bcl-2可能参与了调控喉鳞状细胞癌的发生、发展过程,survivin的高表达可能提示喉癌患者预后不良。survivin蛋白表达与喉鳞癌组织中Bcl-2蛋白的异常表达密切相关。

[Objective] To study the significance of expression of survivin in laryngeal squamous cell carcinoma （LSCC） and its relationship with the expression of Bcl-2 gene, [Methods] The expression of survivin and Bcl-2 were detected using SP immunohistochemistry technique in 47 cases of tumor tissues. Their results were compared to that in adjacent of cancer and normal tissues. [Results] Percentage of survivin expression was 59.6% （28/47）, 25.5% （12/47） in tumor tissues and adjacent tissues respectively. In contrast, normal laryngeal tissues did not express survivin. Over expression of survivin was related to the tumor grade, clinical stage and cervical nodal metastasis. Survivin positive cases were strongly associated with the expression of Bcl-2. [Conclusion] Bcl-2 may be closely correlated with tumor igenesis. High-expression of survivin indicates poor prognosis. Survivin could be a new diagnostic/therapeutic target in LSCC.